Use of nile red for the rapid in situ quantitation of lipids on thin-layer chromatograms
We describe the use of the fluorescent dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, as a general-purpose reagent for the rapid detection and quantitation of a wide variety of lipids and other hydrophobic compounds separated by thin-layer chromatography. After samples are applied to...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1987-10-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520386120 |