Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease
BackgroundInfections are the major cause of morbidity and mortality in patients with primary immunodeficiency disease (PID). Timely and accurate microbiological diagnosis is particularly important in these patients. Metagenomic next-generation sequencing (mNGS) has been used for pathogen detection r...
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| Format: | Article |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.696403/full |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Wenjing Tang Yu Zhang Chong Luo Lina Zhou Lina Zhou Lina Zhou Lina Zhou Zhiyong Zhang Xuemei Tang Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Yunfei An Yunfei An Yunfei An Yunfei An Yunfei An |
| spellingShingle |
Wenjing Tang Yu Zhang Chong Luo Lina Zhou Lina Zhou Lina Zhou Lina Zhou Zhiyong Zhang Xuemei Tang Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Yunfei An Yunfei An Yunfei An Yunfei An Yunfei An Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease Frontiers in Immunology metagenomic next-generation sequencing infection diagnosis primary immunodeficiency disease conventional microbiological tests |
| author_facet |
Wenjing Tang Yu Zhang Chong Luo Lina Zhou Lina Zhou Lina Zhou Lina Zhou Zhiyong Zhang Xuemei Tang Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Xiaodong Zhao Yunfei An Yunfei An Yunfei An Yunfei An Yunfei An |
| author_sort |
Wenjing Tang |
| title |
Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease |
| title_short |
Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease |
| title_full |
Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease |
| title_fullStr |
Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease |
| title_full_unstemmed |
Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency Disease |
| title_sort |
clinical application of metagenomic next-generation sequencing for suspected infections in patients with primary immunodeficiency disease |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Immunology |
| issn |
1664-3224 |
| publishDate |
2021-08-01 |
| description |
BackgroundInfections are the major cause of morbidity and mortality in patients with primary immunodeficiency disease (PID). Timely and accurate microbiological diagnosis is particularly important in these patients. Metagenomic next-generation sequencing (mNGS) has been used for pathogen detection recently. However, few reports describe the use of mNGS for pathogen identification in patients with PID.ObjectiveTo evaluate the utility of mNGS for detecting pathogens in patients with PID, and to compare it with conventional microbiological tests (CMT).MethodsThis single center retrospective study investigated the diagnostic performance of mNGS for pathogens detection in PID patients and compared it with CMT. Sixteen PID patients with suspected infection were enrolled, and medical records were analyzed to extract detailed clinical characteristics such as gene variation, immune status, microbial distribution, time-consuming of mNGS and CMT, treatment, and outcomes.ResultsmNGS identified pathogenic microbe in 93.75% samples, compared to 31.25% for culture and 68.75% for conventional methods, and detected an extra 18 pathogenic microorganisms including rare opportunistic pathogens and Mycobacterium tuberculosis. Pathogen identification by mNGS required 48 hours, compared with bacterial culture for 3-7 days and even longer for fungus and Mycobacterium tuberculosis culture.ConclusionsmNGS has marked advantages over conventional methods for pathogenic diagnosis, particularly opportunistic pathogens and mixed infections, in patients with PID. This method might enable clinicians to make more timely and targeted therapeutic decisions, thereby improving the prognosis of these patients. |
| topic |
metagenomic next-generation sequencing infection diagnosis primary immunodeficiency disease conventional microbiological tests |
| url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.696403/full |
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doaj-f300d631dfdd40e88f00f4284df4e67c2021-08-13T11:34:36ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-08-011210.3389/fimmu.2021.696403696403Clinical Application of Metagenomic Next-Generation Sequencing for Suspected Infections in Patients With Primary Immunodeficiency DiseaseWenjing Tang0Yu Zhang1Chong Luo2Lina Zhou3Lina Zhou4Lina Zhou5Lina Zhou6Zhiyong Zhang7Xuemei Tang8Xiaodong Zhao9Xiaodong Zhao10Xiaodong Zhao11Xiaodong Zhao12Xiaodong Zhao13Yunfei An14Yunfei An15Yunfei An16Yunfei An17Yunfei An18Department of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChina International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Child Infection and Immunity, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChina International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Child Infection and Immunity, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChina International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Child Infection and Immunity, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaBackgroundInfections are the major cause of morbidity and mortality in patients with primary immunodeficiency disease (PID). Timely and accurate microbiological diagnosis is particularly important in these patients. Metagenomic next-generation sequencing (mNGS) has been used for pathogen detection recently. However, few reports describe the use of mNGS for pathogen identification in patients with PID.ObjectiveTo evaluate the utility of mNGS for detecting pathogens in patients with PID, and to compare it with conventional microbiological tests (CMT).MethodsThis single center retrospective study investigated the diagnostic performance of mNGS for pathogens detection in PID patients and compared it with CMT. Sixteen PID patients with suspected infection were enrolled, and medical records were analyzed to extract detailed clinical characteristics such as gene variation, immune status, microbial distribution, time-consuming of mNGS and CMT, treatment, and outcomes.ResultsmNGS identified pathogenic microbe in 93.75% samples, compared to 31.25% for culture and 68.75% for conventional methods, and detected an extra 18 pathogenic microorganisms including rare opportunistic pathogens and Mycobacterium tuberculosis. Pathogen identification by mNGS required 48 hours, compared with bacterial culture for 3-7 days and even longer for fungus and Mycobacterium tuberculosis culture.ConclusionsmNGS has marked advantages over conventional methods for pathogenic diagnosis, particularly opportunistic pathogens and mixed infections, in patients with PID. This method might enable clinicians to make more timely and targeted therapeutic decisions, thereby improving the prognosis of these patients.https://www.frontiersin.org/articles/10.3389/fimmu.2021.696403/fullmetagenomic next-generation sequencinginfectiondiagnosisprimary immunodeficiency diseaseconventional microbiological tests |