Plasma xanthine oxidoreductase activity in Japanese patients with type 2 diabetes across hospitalized treatment

• Main Authors: Yusuke Kawachi, Yuya Fujishima, Hitoshi Nishizawa, Hirofumi Nagao, Takashi Nakamura, Seigo Akari, Takayo Murase, Naohiro Taya, Kazuo Omori, Akimitsu Miyake, Shiro Fukuda, Mitsuyoshi Takahara, Shunbun Kita, Naoto Katakami, Norikazu Maeda, Iichiro Shimomura
• Format: Article
• Language: English
• Published: Wiley 2021-08-01
• Series: Journal of Diabetes Investigation
• Subjects: Liver transaminases; Type 2 diabetes mellitus; Xanthine oxidoreductase
• Online Access: https://doi.org/10.1111/jdi.13467

Abstract Aims/Introduction Xanthine oxidoreductase (XOR) is an enzyme that catalyzes hypoxanthine and xanthine to xanthine and uric acid, respectively. Plasma XOR activity has recently been measured in humans. However, limited information is known about plasma XOR activity in patients with type 2 diabetes mellitus, and its changes after short‐term glycemic control treatment. Materials and Methods We enrolled 28 Japanese patients (10 men/18 women) with type 2 diabetes mellitus who were hospitalized to undergo medical treatment for diabetes. Plasma XOR activity, quantified using triple quadrupole mass spectrometry and liquid chromatography, and other clinical parameters were examined at admission and 2 weeks after treatment during hospitalization. Changes in plasma XOR activity after treatment during hospitalization and associated clinical parameters were assessed. Results At the time of admission, the median plasma XOR activity was 83.1 pmol/h/mL, with a wide range of 14.4–1150 pmol/h/mL. Multiple regression analysis identified serum aspartate transaminase and alanine transaminase levels as significant and independent factors correlating with the baseline plasma XOR. Two weeks of treatment during hospitalization was associated with a significant decrease in plasma XOR activity. Changes in serum aspartate transaminase were also the only significant and independent factor correlating with changes in plasma XOR activity. Conclusions A close relationship was observed between plasma XOR activity and liver transaminases in patients with type 2 diabetes mellitus, cross‐sectionally, and also across treatment during hospitalization.