Top-Down N-Doped Carbon Quantum Dots for Multiple Purposes: Heavy Metal Detection and Intracellular Fluorescence

• Main Authors: Francesca Limosani, Elvira Maria Bauer, Daniele Cecchetti, Stefano Biagioni, Viviana Orlando, Roberto Pizzoferrato, Paolo Prosposito, Marilena Carbone
• Format: Article
• Language: English
• Published: MDPI AG 2021-08-01
• Series: Nanomaterials
• Subjects: fullerene; carbon quantum dots; heavy metals; photoluminescence; bioimaging; quenching
• Online Access: https://www.mdpi.com/2079-4991/11/9/2249
• ISSN: 2079-4991

In the present study, we successfully synthesized N-doped carbon quantum dots (N-CQDs) using a top-down approach, i.e., hydroxyl radical opening of fullerene with hydrogen peroxide, in basic ambient using ammonia for two different reaction times. The ensuing characterization via dynamic light scattering, SEM, and IR spectroscopy revealed a size control that was dependent on the reaction time, as well as a more pronounced -NH₂ functionalization. The N-CQDs were probed for metal ion detection in aqueous solutions and during bioimaging and displayed a Cr³⁺ and Cu²⁺ selectivity shift at a higher degree of -NH₂ functionalization, as well as HEK-293 cell nuclei marking.