Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer
The utility of circular RNAs (circRNAs) as molecular biomarkers has recently emerged. However, only a handful of them have already been studied in colorectal cancer (CRC). The purpose of this study was to identify new circRNAs deriving from <i>BCL2L12</i>, a member of the BCL2 apoptosis-...
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doaj-f2600d0afedc428989fd54cc7dd0ab942020-11-25T04:11:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-01218867886710.3390/ijms21228867Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal CancerParaskevi Karousi0Pinelopi I. Artemaki1Christina D. Sotiropoulou2Spyridon Christodoulou3Andreas Scorilas4Christos K. Kontos5Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceFourth Surgery Department, University General Hospital “Attikon”, 12462 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceThe utility of circular RNAs (circRNAs) as molecular biomarkers has recently emerged. However, only a handful of them have already been studied in colorectal cancer (CRC). The purpose of this study was to identify new circRNAs deriving from <i>BCL2L12</i>, a member of the BCL2 apoptosis-related family, and investigate their potential as biomarkers in CRC. Total RNA extracts from CRC cell lines and tissue samples were reversely transcribed. By combining PCR with divergent primers and nested PCR followed by Sanger sequencing, we were able to discover two <i>BCL2L12</i> circRNAs. Subsequently, bioinformatical tools were used to predict the interactions of these circRNAs with microRNAs (miRNAs) and RNA-binding proteins (RBPs). Following a PCR-based pre-amplification, real-time qPCR was carried out for the quantification of each circRNA in CRC samples and cell lines. Biostatistical analysis was used to assess their potential prognostic value in CRC. Both novel <i>BCL2L12</i> circRNAs likely interact with particular miRNAs and RBPs. Interestingly, circ-BCL2L12-2 expression is inversely associated with TNM stage, while circ-BCL2L12-1 overexpression is associated with shorter overall survival in CRC, particularly among TNM stage II patients. Overall, we identified two novel <i>BCL2L12</i> circRNAs, one of which can further stratify TNM stage II patients into two subgroups with substantially distinct prognosis.https://www.mdpi.com/1422-0067/21/22/8867circRNAsalternative splicingtranscriptomicsmiRNA spongesRNA-binding proteinsapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paraskevi Karousi Pinelopi I. Artemaki Christina D. Sotiropoulou Spyridon Christodoulou Andreas Scorilas Christos K. Kontos |
spellingShingle |
Paraskevi Karousi Pinelopi I. Artemaki Christina D. Sotiropoulou Spyridon Christodoulou Andreas Scorilas Christos K. Kontos Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer International Journal of Molecular Sciences circRNAs alternative splicing transcriptomics miRNA sponges RNA-binding proteins apoptosis |
author_facet |
Paraskevi Karousi Pinelopi I. Artemaki Christina D. Sotiropoulou Spyridon Christodoulou Andreas Scorilas Christos K. Kontos |
author_sort |
Paraskevi Karousi |
title |
Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer |
title_short |
Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer |
title_full |
Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer |
title_fullStr |
Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer |
title_full_unstemmed |
Identification of Two Novel Circular RNAs Deriving from <i>BCL2L12</i> and Investigation of Their Potential Value as a Molecular Signature in Colorectal Cancer |
title_sort |
identification of two novel circular rnas deriving from <i>bcl2l12</i> and investigation of their potential value as a molecular signature in colorectal cancer |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-11-01 |
description |
The utility of circular RNAs (circRNAs) as molecular biomarkers has recently emerged. However, only a handful of them have already been studied in colorectal cancer (CRC). The purpose of this study was to identify new circRNAs deriving from <i>BCL2L12</i>, a member of the BCL2 apoptosis-related family, and investigate their potential as biomarkers in CRC. Total RNA extracts from CRC cell lines and tissue samples were reversely transcribed. By combining PCR with divergent primers and nested PCR followed by Sanger sequencing, we were able to discover two <i>BCL2L12</i> circRNAs. Subsequently, bioinformatical tools were used to predict the interactions of these circRNAs with microRNAs (miRNAs) and RNA-binding proteins (RBPs). Following a PCR-based pre-amplification, real-time qPCR was carried out for the quantification of each circRNA in CRC samples and cell lines. Biostatistical analysis was used to assess their potential prognostic value in CRC. Both novel <i>BCL2L12</i> circRNAs likely interact with particular miRNAs and RBPs. Interestingly, circ-BCL2L12-2 expression is inversely associated with TNM stage, while circ-BCL2L12-1 overexpression is associated with shorter overall survival in CRC, particularly among TNM stage II patients. Overall, we identified two novel <i>BCL2L12</i> circRNAs, one of which can further stratify TNM stage II patients into two subgroups with substantially distinct prognosis. |
topic |
circRNAs alternative splicing transcriptomics miRNA sponges RNA-binding proteins apoptosis |
url |
https://www.mdpi.com/1422-0067/21/22/8867 |
work_keys_str_mv |
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