A multiplex two-color real-time PCR method for quality-controlled molecular diagnostic testing of FFPE samples.

Reverse transcription quantitative real-time PCR (RT-qPCR) tests support personalized cancer treatment through more clinically meaningful diagnosis. However, samples obtained through standard clinical pathology procedures are formalin-fixed, paraffin-embedded (FFPE) and yield small samples with low...

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Bibliographic Details
Main Authors: Jiyoun Yeo, Erin L Crawford, Thomas M Blomquist, Lauren M Stanoszek, Rachel E Dannemiller, Jill Zyrek, Luis E De Las Casas, Sadik A Khuder, James C Willey
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3931751?pdf=render