| Summary: | Microbial metabolomics is a challenge strategy that allows a comprehensive analysis of metabolites within a microorganism and may support a new approach in microbial research, including the microbial diagnosis. Thus, the aim of this research was to in-depth explore a metabolomics strategy based on the use of an advanced multidimensional gas chromatography for the comprehensive mapping of cellular metabolites of <i>C. albicans</i> and non-<i>C. albicans</i> (<i>C. glabrata</i> and <i>C. tropicalis</i>) and therefore contributing for the development of a comprehensive platform for fungal detection management and for species distinction in early growth times (6 h). The volatile fraction comprises 126 putatively identified metabolites distributed over several chemical families: acids, alcohols, aldehydes, hydrocarbons, esters, ketones, monoterpenic and sesquiterpenic compounds, norisoprenoids, phenols and sulphur compounds. These metabolites may be related with different metabolic pathways, such as amino acid metabolism and biosynthesis, fatty acids metabolism, aromatic compounds degradation, mono and sesquiterpenoid synthesis and carotenoid cleavage. These results represent an enlargement of <i>ca</i>. 70% of metabolites not previously reported for <i>C. albicans</i>, 91% for <i>C. glabrata</i> and 90% for <i>C. tropicalis</i>. This study represents the most detailed study about <i>Candida</i> species exometabolome, allowing a metabolomic signature of each species, which signifies an improvement towards the construction of a <i>Candida</i> metabolomics platform whose application in clinical diagnostics can be crucial to guide therapeutic interventions.
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