A Cas-embedding strategy for minimizing off-target effects of DNA base editors

DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.

Bibliographic Details
Main Authors:	Yajing Liu, Changyang Zhou, Shisheng Huang, Lu Dang, Yu Wei, Jun He, Yingsi Zhou, Shaoshuai Mao, Wanyu Tao, Yu Zhang, Hui Yang, Xingxu Huang, Tian Chi
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-19690-0

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spelling	doaj-f1fdbef8038d4a33ade4dc6a223c19e32021-05-11T08:14:28ZengNature Publishing GroupNature Communications2041-17232020-11-011111910.1038/s41467-020-19690-0A Cas-embedding strategy for minimizing off-target effects of DNA base editorsYajing Liu0Changyang Zhou1Shisheng Huang2Lu Dang3Yu Wei4Jun He5Yingsi Zhou6Shaoshuai Mao7Wanyu Tao8Yu Zhang9Hui Yang10Xingxu Huang11Tian Chi12School of Life Science and Technology, ShanghaiTech UniversityInstitute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesSchool of Life Science and Technology, ShanghaiTech UniversityCancer Hospital and Institute of Guangzhou Medical UniversityInstitute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesSchool of Life Science and Technology, ShanghaiTech UniversityInstitute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesInstitute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesSchool of Life Science and Technology, ShanghaiTech UniversitySchool of Life Science and Technology, ShanghaiTech UniversityInstitute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesSchool of Life Science and Technology, ShanghaiTech UniversitySchool of Life Science and Technology, ShanghaiTech UniversityDNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.https://doi.org/10.1038/s41467-020-19690-0
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yajing Liu Changyang Zhou Shisheng Huang Lu Dang Yu Wei Jun He Yingsi Zhou Shaoshuai Mao Wanyu Tao Yu Zhang Hui Yang Xingxu Huang Tian Chi
spellingShingle	Yajing Liu Changyang Zhou Shisheng Huang Lu Dang Yu Wei Jun He Yingsi Zhou Shaoshuai Mao Wanyu Tao Yu Zhang Hui Yang Xingxu Huang Tian Chi A Cas-embedding strategy for minimizing off-target effects of DNA base editors Nature Communications
author_facet	Yajing Liu Changyang Zhou Shisheng Huang Lu Dang Yu Wei Jun He Yingsi Zhou Shaoshuai Mao Wanyu Tao Yu Zhang Hui Yang Xingxu Huang Tian Chi
author_sort	Yajing Liu
title	A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_short	A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_full	A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_fullStr	A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_full_unstemmed	A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_sort	cas-embedding strategy for minimizing off-target effects of dna base editors
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-11-01
description	DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.
url	https://doi.org/10.1038/s41467-020-19690-0
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A Cas-embedding strategy for minimizing off-target effects of DNA base editors

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