Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concent...

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Main Authors:	Polina A. Kameneva, Kseniya V. Efimova, Viacheslav G. Rybin, Tatiana Y. Orlova
Format:	Article
Language:	English
Published:	MDPI AG 2015-09-01
Series:	Toxins
Subjects:	Prorocentrum foraminosum DTX-1 molecular identification HPLC-HRMS Russia
Online Access:	http://www.mdpi.com/2072-6651/7/10/3947

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spelling	doaj-f1ce6fcdada44549a233bd035f90cbb62020-11-24T21:13:24ZengMDPI AGToxins2072-66512015-09-017103947395910.3390/toxins7103947toxins7103947Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of JapanPolina A. Kameneva0Kseniya V. Efimova1Viacheslav G. Rybin2Tatiana Y. Orlova3Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaFor the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.http://www.mdpi.com/2072-6651/7/10/3947Prorocentrum foraminosumDTX-1molecular identificationHPLC-HRMSRussia
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova
spellingShingle	Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan Toxins Prorocentrum foraminosum DTX-1 molecular identification HPLC-HRMS Russia
author_facet	Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova
author_sort	Polina A. Kameneva
title	Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_short	Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_full	Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_fullStr	Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_full_unstemmed	Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_sort	detection of dinophysistoxin-1 in clonal culture of marine dinoflagellate prorocentrum foraminosum (faust m.a., 1993) from the sea of japan
publisher	MDPI AG
series	Toxins
issn	2072-6651
publishDate	2015-09-01
description	For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.
topic	Prorocentrum foraminosum DTX-1 molecular identification HPLC-HRMS Russia
url	http://www.mdpi.com/2072-6651/7/10/3947
work_keys_str_mv	AT polinaakameneva detectionofdinophysistoxin1inclonalcultureofmarinedinoflagellateprorocentrumforaminosumfaustma1993fromtheseaofjapan AT kseniyavefimova detectionofdinophysistoxin1inclonalcultureofmarinedinoflagellateprorocentrumforaminosumfaustma1993fromtheseaofjapan AT viacheslavgrybin detectionofdinophysistoxin1inclonalcultureofmarinedinoflagellateprorocentrumforaminosumfaustma1993fromtheseaofjapan AT tatianayorlova detectionofdinophysistoxin1inclonalcultureofmarinedinoflagellateprorocentrumforaminosumfaustma1993fromtheseaofjapan
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Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

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