Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation

Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation

The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A +, was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detecte...

Full description

Bibliographic Details
Main Authors: Mohamed Houssaini-Iraqui, Naima Khamlichi, Jamal El Yamani, Nalin Rastogi
Format: Article
Language:English
Published: Hindawi Limited 2001-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/S1110724301000225
id doaj-f1c7e4d13c97433aa5af60a73cfd4eb2
record_format Article
spelling doaj-f1c7e4d13c97433aa5af60a73cfd4eb22020-11-24T21:45:45ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512001-01-0112798410.1155/S1110724301000225Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV RadiationMohamed Houssaini-Iraqui0Naima Khamlichi1Jamal El Yamani2Nalin Rastogi3Biotechnology Laboratory, Sidi Mohammed Ben Abdellah University, FST, P.O. Box 2202, Fez, MoroccoBiotechnology Laboratory, Sidi Mohammed Ben Abdellah University, FST, P.O. Box 2202, Fez, MoroccoBiotechnology Laboratory, Sidi Mohammed Ben Abdellah University, FST, P.O. Box 2202, Fez, MoroccoTuberculosis and Mycobacteria unit, Pasteur Institut, P.O. Box 484, Pointe a Pitre, GuadeloupeThe plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A +, was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.http://dx.doi.org/10.1155/S1110724301000225
collection DOAJ
language English
format Article
sources DOAJ
author Mohamed Houssaini-Iraqui
Naima Khamlichi
Jamal El Yamani
Nalin Rastogi
spellingShingle Mohamed Houssaini-Iraqui
Naima Khamlichi
Jamal El Yamani
Nalin Rastogi
Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
Journal of Biomedicine and Biotechnology
author_facet Mohamed Houssaini-Iraqui
Naima Khamlichi
Jamal El Yamani
Nalin Rastogi
author_sort Mohamed Houssaini-Iraqui
title Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
title_short Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
title_full Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
title_fullStr Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
title_full_unstemmed Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation
title_sort response of escherichia coli containing mycobacterial carotene genes to uv radiation
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2001-01-01
description The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A +, was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.
url http://dx.doi.org/10.1155/S1110724301000225
work_keys_str_mv AT mohamedhoussainiiraqui responseofescherichiacolicontainingmycobacterialcarotenegenestouvradiation
AT naimakhamlichi responseofescherichiacolicontainingmycobacterialcarotenegenestouvradiation
AT jamalelyamani responseofescherichiacolicontainingmycobacterialcarotenegenestouvradiation
AT nalinrastogi responseofescherichiacolicontainingmycobacterialcarotenegenestouvradiation
_version_ 1725904444756852736

Similar Items