Uso de fibrinógeno humano en la generación de soportes para la obtención de equivalentes tisulares

• Main Authors: Dionisio Malagón, Carmen Cardozo, Rubén Godoy
• Format: Article
• Language: Spanish
• Published: Universidad Nacional de Colombia 2012-06-01
• Series: Revista Colombiana de Biotecnología
• Subjects: fibrin; culture cells; biomaterial; hydrogel; protein; fibrina; cultivos celulares; hidrogel; proteína
• Online Access: http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/28029

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table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} --> <!--[endif] --> <p class="MsoNormal" style="text-align: justify;"><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">Título en ingles: Use of</span></strong></span><span class="longtext"><strong><span style="font-size: 10pt;" lang="EN-US"> </span></strong></span><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">human fibrinogen</span></strong></span><span class="longtext"><strong><span style="font-size: 10pt;" lang="EN-US"> </span></strong></span><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">in the generation</span></strong></span><span class="longtext"><strong><span style="font-size: 10pt;" lang="EN-US"> </span></strong></span><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">of scaffold for obtaining</span></strong></span><span class="longtext"><strong><span style="font-size: 10pt;" lang="EN-US"> T</span></strong></span><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">issue</span></strong></span><span class="longtext"><strong><span style="font-size: 10pt;" lang="EN-US"> </span></strong></span><span class="hps"><strong><span style="font-size: 10pt;" lang="EN-US">equivalents</span></strong></span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="font-size: 10pt;" lang="ES">Resumen</span></strong></p> <p class="MsoNormal" style="text-align: justify;"><span style="font-size: 10pt;" lang="ES">Desde sus orígenes, la Ingeniería de Tejidos ha buscado diversos materiales que puedan ser utilizados para la generación de soportes que sirvan para el anclaje, proliferación y diferenciación celular que conduzcan a la obtención de tejidos humanos. Muchos materiales de tipo cerámico, polimérico y metálico se han evaluado, pero hasta la fecha muchos de ellos han sido rechazados por diversas razones, entre otras su escasa biocompatibilidad y biodegradabilidad, la respuesta inmune generada, la baja resistencia mecánica o el riesgo de transmisión de virus o priones. El fibrinógeno es una proteína presente en el plasma sanguíneo que puede ser utilizada para la generación de soportes tridimensionales que favorezcan el crecimiento de células; se obtiene a partir del propio paciente, bancos de sangre o como proteína purificada (Tisseel® o Tissucol®, Laboratorios Baxter). El fibrinógeno evita el desencadenamiento de una respuesta inmunológica y el uso de productos xenogénicos. Debido a la estructura proteica, la adhesión y proliferación celular se ven favorecidas dando excelentes resultados en la generación de equivalentes de piel, cartílago, córnea y reemplazos cardiacos en aplicaciones <em>in vitro</em> e <em>in vivo</em>. Como desventajas presenta su rápida degradación y su baja resistencia mecánica; sin embargo, en los últimos años se han venido evaluando mezclas con algunos biopolímeros como ácido poliláctico (PLLA), ácido poli-glicólico (PGA) y alginato de sodio. Esta revisión presenta algunas de las principales aplicaciones del fibrinógeno en Ingeniería de Tejidos.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="font-size: 10pt;" lang="ES">Palabras clave:</span></strong><span style="font-size: 10pt;" lang="ES"> fibrina, cultivos celulares, biomaterial, hidrogel, proteína.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="font-size: 10pt;" lang="EN-US">Abstract</span></strong></p> <p class="MsoNormal" style="text-align: justify;"><span class="longtext"><span style="font-size: 10pt; background: none repeat scroll 0% 0% white;" lang="EN-US">Since its origin, Tissue Engineering has sought various materials that can be used for generation of scaffolds that serve to anchor, proliferation and cell differentiation leading to the production of human tissues. Many materials such as ceramic, polymeric and metal type have been evaluated to date but many have been rejected for various reasons, including its limited biocompatibility and biodegradability, immune response generated, low mechanical strength or the risk of transmission of </span></span><span class="longtext"><span style="font-size: 10pt;" lang="EN-US">virus or prions. <span style="background: none repeat scroll 0% 0% white;">Fibrinogen is a protein present in blood plasma that can be used to generate three-dimensional scaffolds that favors growth of cells, it is obtained from the patient itself, bank of blood or purified protein (Tisseel® or Tissucol®, Laboratorios Baxter). Fibrinogen acts slowing or reversing the immune response and avoiding the use of xenogeneic materials. Because the protein structure, adhesion and cell proliferation is favored with excellent results in the generation of skin equivalents, cartilage, cornea and even heart replacements <em>in vitro</em> and <em>in vivo</em>. The disadvantages presented are the rapid degradation and low mechanical strength, but in recent years it has been evaluating some biopolymer mixtures as polylactic acid (PLLA), poly-glycolic acid (PGA) and sodium alginate. This review presents some of the main applications of fibrinogen in Tissue Engineering. </span></span></span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="font-size: 10pt;" lang="EN-US">Key words:</span></strong><span style="font-size: 10pt;" lang="EN-US"> fibrin, culture cells, biomaterial, hydrogel, protein.</span></p>