λ Recombineering Used to Engineer the Genome of Phage T7

Bacteriophage T7 and T7-like bacteriophages are valuable genetic models for lytic phage biology that have heretofore been intractable with in vivo genetic engineering methods. This manuscript describes that the presence of λ Red recombination proteins makes in vivo recombineering of T7 possible, so...

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Bibliographic Details
Main Authors: Jordan D. Jensen, Adam R. Parks, Sankar Adhya, Alison J. Rattray, Donald L. Court
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Antibiotics
Subjects:
Online Access:https://www.mdpi.com/2079-6382/9/11/805