3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration
Current strategies for regeneration of large bone fractures yield limited clinical success mainly due to poor integration and healing. Multidisciplinary approaches in design and development of functional tissue engineered scaffolds are required to overcome these translational challenges. Here, a new...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/7/1099 |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mohammadreza Shokouhimehr Andrea S. Theus Archana Kamalakar Liqun Ning Cong Cao Martin L. Tomov Jarred M. Kaiser Steven Goudy Nick J. Willett Ho Won Jang Christopher N. LaRock Philip Hanna Aron Lechtig Mohamed Yousef Janaina Da Silva Martins Ara Nazarian Mitchel B. Harris Morteza Mahmoudi Vahid Serpooshan |
| spellingShingle |
Mohammadreza Shokouhimehr Andrea S. Theus Archana Kamalakar Liqun Ning Cong Cao Martin L. Tomov Jarred M. Kaiser Steven Goudy Nick J. Willett Ho Won Jang Christopher N. LaRock Philip Hanna Aron Lechtig Mohamed Yousef Janaina Da Silva Martins Ara Nazarian Mitchel B. Harris Morteza Mahmoudi Vahid Serpooshan 3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration Polymers damage-specific scaffold bone 3D bioprinting tissue engineering hyperelastic bone superparamagnetic iron oxide nanoparticles antibacterial |
| author_facet |
Mohammadreza Shokouhimehr Andrea S. Theus Archana Kamalakar Liqun Ning Cong Cao Martin L. Tomov Jarred M. Kaiser Steven Goudy Nick J. Willett Ho Won Jang Christopher N. LaRock Philip Hanna Aron Lechtig Mohamed Yousef Janaina Da Silva Martins Ara Nazarian Mitchel B. Harris Morteza Mahmoudi Vahid Serpooshan |
| author_sort |
Mohammadreza Shokouhimehr |
| title |
3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration |
| title_short |
3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration |
| title_full |
3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration |
| title_fullStr |
3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration |
| title_full_unstemmed |
3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration |
| title_sort |
3d bioprinted bacteriostatic hyperelastic bone scaffold for damage-specific bone regeneration |
| publisher |
MDPI AG |
| series |
Polymers |
| issn |
2073-4360 |
| publishDate |
2021-03-01 |
| description |
Current strategies for regeneration of large bone fractures yield limited clinical success mainly due to poor integration and healing. Multidisciplinary approaches in design and development of functional tissue engineered scaffolds are required to overcome these translational challenges. Here, a new generation of hyperelastic bone (HB) implants, loaded with superparamagnetic iron oxide nanoparticles (SPIONs), are 3D bioprinted and their regenerative effect on large non-healing bone fractures is studied. Scaffolds are bioprinted with the geometry that closely correspond to that of the bone defect, using an osteoconductive, highly elastic, surgically friendly bioink mainly composed of hydroxyapatite. Incorporation of SPIONs into HB bioink results in enhanced bacteriostatic properties of bone grafts while exhibiting no cytotoxicity. In vitro culture of mouse embryonic cells and human osteoblast-like cells remain viable and functional up to 14 days on printed HB scaffolds. Implantation of damage-specific bioprinted constructs into a rat model of femoral bone defect demonstrates significant regenerative effect over the 2-week time course. While no infection, immune rejection, or fibrotic encapsulation is observed, HB grafts show rapid integration with host tissue, ossification, and growth of new bone. These results suggest a great translational potential for 3D bioprinted HB scaffolds, laden with functional nanoparticles, for hard tissue engineering applications. |
| topic |
damage-specific scaffold bone 3D bioprinting tissue engineering hyperelastic bone superparamagnetic iron oxide nanoparticles antibacterial |
| url |
https://www.mdpi.com/2073-4360/13/7/1099 |
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doaj-f11e1a4be88c40f8aba081a0d42b44f12021-03-30T23:03:50ZengMDPI AGPolymers2073-43602021-03-01131099109910.3390/polym130710993D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone RegenerationMohammadreza Shokouhimehr0Andrea S. Theus1Archana Kamalakar2Liqun Ning3Cong Cao4Martin L. Tomov5Jarred M. Kaiser6Steven Goudy7Nick J. Willett8Ho Won Jang9Christopher N. LaRock10Philip Hanna11Aron Lechtig12Mohamed Yousef13Janaina Da Silva Martins14Ara Nazarian15Mitchel B. Harris16Morteza Mahmoudi17Vahid Serpooshan18Department of Materials Science and Engineering, Research Institute of Advanced Materials, Seoul National University, Seoul 08826, KoreaDepartment of Biomedical Engineering, Georgia Institute of Technology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Otolaryngology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Georgia Institute of Technology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Physics, Emory University, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Georgia Institute of Technology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Orthopedics, Emory University, Atlanta, GA 30322, USADepartment of Otolaryngology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Georgia Institute of Technology, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Materials Science and Engineering, Research Institute of Advanced Materials, Seoul National University, Seoul 08826, KoreaDepartment of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, GA 30322, USACenter for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USACenter for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Orthopedic Surgery, Sohag University, Sohag 82524, EgyptEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, 50 Blossom St, Thier 11, Boston, MA 02114, USACenter for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USAOrthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAPrecision Health Program & Department of Radiology, Michigan State University, East Lansing, MI 48824, USADepartment of Biomedical Engineering, Georgia Institute of Technology, School of Medicine, Emory University, Atlanta, GA 30322, USACurrent strategies for regeneration of large bone fractures yield limited clinical success mainly due to poor integration and healing. Multidisciplinary approaches in design and development of functional tissue engineered scaffolds are required to overcome these translational challenges. Here, a new generation of hyperelastic bone (HB) implants, loaded with superparamagnetic iron oxide nanoparticles (SPIONs), are 3D bioprinted and their regenerative effect on large non-healing bone fractures is studied. Scaffolds are bioprinted with the geometry that closely correspond to that of the bone defect, using an osteoconductive, highly elastic, surgically friendly bioink mainly composed of hydroxyapatite. Incorporation of SPIONs into HB bioink results in enhanced bacteriostatic properties of bone grafts while exhibiting no cytotoxicity. In vitro culture of mouse embryonic cells and human osteoblast-like cells remain viable and functional up to 14 days on printed HB scaffolds. Implantation of damage-specific bioprinted constructs into a rat model of femoral bone defect demonstrates significant regenerative effect over the 2-week time course. While no infection, immune rejection, or fibrotic encapsulation is observed, HB grafts show rapid integration with host tissue, ossification, and growth of new bone. These results suggest a great translational potential for 3D bioprinted HB scaffolds, laden with functional nanoparticles, for hard tissue engineering applications.https://www.mdpi.com/2073-4360/13/7/1099damage-specific scaffoldbone 3D bioprintingtissue engineeringhyperelastic bonesuperparamagnetic iron oxide nanoparticlesantibacterial |