Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

• Main Authors: Rajeev Jain, Ramkishor Sharma
• Format: Article
• Language: English
• Published: Elsevier 2012-04-01
• Series: Journal of Pharmaceutical Analysis
• Online Access: http://www.sciencedirect.com/science/article/pii/S2095177911000438

Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in BrittonâRobinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range â1.2 to â1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring. Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma. The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50â5.00 μg/mL with the detection limit 0.17 μg/mL, whereas in pharmaceutical formulation 0.25â2.25 μg/mL with the detection limit 0.08 μg/mL. Keywords: Voltammetry, Adefovir, Solid-phase extraction, Protein-precipitation, Human plasma, Pharmaceutical formulation