Purification and characterization of lipase by Bacillus methylotrophicus PS3 under submerged fermentation and its application in detergent industry

• Main Authors: Pushpinder Sharma, Nivedita Sharma, Shruti Pathania, Shweta Handa
• Format: Article
• Language: English
• Published: SpringerOpen 2017-12-01
• Series: Journal of Genetic Engineering and Biotechnology
• Subjects: Lipase; Bacillus methylotrophicus Ps3; 16s rRNA; Purification; Characterization; Detergent
• Online Access: http://www.sciencedirect.com/science/article/pii/S1687157X17300355

Lipase production bacterial isolate was isolated from soil of service station and identified as Bacillus methylotrophicus PS3 by 16SrRNA with accession number |LN999829.1|. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatography. The molecular weight of purified enzyme was 31.40 kDa on SDS-PAGE. This purification procedure resulted in 2.90-fold purification of lipase with a 24.10% final yield. The purified lipase presented maximal hydrolytic activity at a temperature of 55 °C, and pH of 7.0. Lipase activity was stimulated by Triton X-100 and SDS with Mg2+ and Ca2+ metals employ a positive effect and outlast its stable in organic solvent i.e. methanol and ethanol.