Research of qualitative composition and quantitative content of fatty acids of Crambe cordifolia Steven and Crambe koktebelica (Junge) N. Busch leaves

• Main Authors: S. M. Marchyshyn, L. I. Stoiko, O. Ya. Skrynchuk, D. B. Rakhmetov
• Format: Article
• Language: English
• Published: Zaporozhye State Medical University 2019-03-01
• Series: Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki
• Subjects: Crambe cordifolia Steven; Crambe koktebelica (Junge) N. Busch; fatty acids; plant leaves; gas chromatography mass spectrometry; α-linolenic acid; linoleic acids; palmitic acid
• Online Access: http://pharmed.zsmu.edu.ua/article/view/158939/158789

The aim of the research was to establish a qualitative composition and to determine the quantitative content of fatty acids of Crambe cordifolia Steven and Crambe koktebelica (Junge) N. Busch leaves. Materials and methods. Leaves of the Crambe cordifolia Steven and Crambe koktebelica (Junge) N. Busch were selected as the objects of the study. The raw materials were provided by the Department of cultural flora of M. Gryshko National Botanic Garden of the National Academy of Sciences of Ukraine. The leaves were collected in summer 2018. The qualitative composition and quantitative content of fatty acids were determined by the method of gas chromatography with mass spectrometry using chromatograph Agilent 6890N with mass detector 5973 inert (Agilent Technologies, USA). Results. Seven fatty acids were found in the Crambe cordifolia Steven leaves, among which four are saturated, two are polyunsaturated, and one is monounsaturated. Quantitatively dominated α-linolenic (9.68 mg/g, 47.87 % of the total content of all identified fatty acids), palmitic (4.88 mg/g, 24.14%), and linoleic (1.84 mg/g; 9.10 %) acids. The other fatty acids were 18.89 %. Among the twelve fatty acids identified in the Crambe koktebelica (Junge) N. Busch leaves, eight belong to saturated, two to polyunsaturated and two to monounsaturated. Quantitatively dominated α-linolenic (8.84 mg/g, 42.95 %), linoleic (2.36 mg/g, 11.47 %), and palmitic (4.53 mg/g, 22.01 %) acids. Conclusions. The qualitative composition and quantitative content of fatty acids were determined in the Crambe cordifolia Steven and the Crambe koktebelica (Junge) N. Busch leaves by gas chromatography with mass spectrometry for the first time. Quantitative content of seven fatty acids was identified and determined in the Crambe cordifolia Steven while in the Crambe koktebelica (Junge) N. Busch were twelve fatty acids. Linoleic and α-linolenic acids dominate in the studied objects.