Antilipase activity guided fractionation of Vinca major

• Main Authors: Sukhdev Singh, Singh Shamsher Kanwar
• Format: Article
• Language: English
• Published: Elsevier 2018-10-01
• Series: Journal of King Saud University: Science
• Online Access: http://www.sciencedirect.com/science/article/pii/S1018364716307571

Methanolic extract of flowers of Vinca major (VFE) reduced the activity of porcine pancreatic lipase (PPL) which was reported in our previous study. To identify the antilipase molecule(s) in the VFE, the antilipase activity-guided fractionation for the purification of lipase inhibitory molecule(s) by Silica Gel and Sephadex G-50 column chromatography was attempted, and the final anti-lipase fraction was examined by ESI-MS and FT-IR spectroscopy. Two molecules namely, Majoridine and Akuammine were elucidated to be present in the flowers of V. major which possessed high PPL inhibition activity. Further, both molecules were looked in silico for their interactions with PPL and other important proteins of lipid metabolism using molecular docking studies. Majoridine and Akuammine demonstrated significant binding interactions with active site residues of PPL. Keywords: Vinca major, Fractionation, ESI-MS, FT-IR, Majoridine, Akuammine, Docking