| Summary: | <p>In this study, hexane, chloroform and water extracts were prepared by making consecutive extraction of <em>Achillea biserrata</em> from the family<em> Asteraceae. </em>Volatile oil of the same types was obtained by water distillation that was continued 4 hours in the Clevenger fixture and dried by adding anhydrous sodium sulfate. After GS/MS analysis, volatile oil of the same types was kept at 4 ºC in the refrigerator and dark until the work activity was performed. Volatile oil yield that was obtained from mixture of all dried flower and plant leaves were determined for <em>Achillea biserrata</em> 0.4%(v/w) The antioxidant activities of extracts, total phenol, flavonoid, proanthocyanidin and anthocyanin amounts were determined. Two different tests were performed for measurements of antioxidant activities.<sup>1</sup> Especially, for the purpose of meeting the general properties of good additives’ free radical cleaning activity was done by DPPH method and the inhibition of oxidation activity was done by with β-carotene method. The 50% inhibition (IC<sub>50</sub>) values of the water, methanol:water and chloroform extracts were 19.6, 37.9 and 114 μg /mL, respectively ın DPPH test. Besıdes, the nonpolar extracts were active in β-carotene/linoleic acid test system. Total phenolics and total flavonoid contents were highest in the water extract possessing 3.39% and 1.12%, respectively. Methanolic extract showed the presence of antioxidant phenolic compounds such as protocatecuic acid (177.83 g/g) and chlorogenic acid (164.78 g/g) ın HPLC analsıs. Automated extraction system obtained better separation of the active principles from plant tissues than conventional extraction procedures.</p>
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