The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
BACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the b...
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doaj-f09fcd61e9304783a882875352aadc4c2020-11-25T00:18:25ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792016-08-01829710210.18585/inabj.v8i2.193196The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus AssayIrene Karlina0Rahmi Amtha1Boedi Oetomo Roeslan2Yuniar Zen3University of TrisaktiUniversity of TrisaktiUniversity Of TrisaktiUniversity of TrisaktiBACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the biocompatibility of stainless steel brackets, micronucleus assay as one of genotoxicity assay is used in this study. To determine the differences and the correlation of metal ions release and genotoxic activity among three brand stainless steel brackets. METHODS: Three brands of brackets were immersed in artificial saliva for 672 hours and the release of ion chromium, nikel and iron were examined. The cytokynesis block micronucleus assay (CBMN) using lymphocytes was performed as well. RESULTS: The highest metal releasing were nickel, cromium, iron, respectively (30.5, 27.2, 23.4 ppb). There was a significant differences between total nickel and iron ion release among three brand brackets (p=0.04, p=0.02). Genotoxicity of metal ion released was correlated with durration of immersion brackets (p=0.01). Genotoxicity was significant correlated with the release of chromium (p=0.03) and nickel (p=0.01). CONCLUSION: Genotoxicity of stainless steel brackets was influenced by duration of immersion but not influenced by brand brackets. KEYWORDS: genotoxicity, stainless steel brackets, metal ionhttp://inabj.org/index.php/ibj/article/view/193 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irene Karlina Rahmi Amtha Boedi Oetomo Roeslan Yuniar Zen |
spellingShingle |
Irene Karlina Rahmi Amtha Boedi Oetomo Roeslan Yuniar Zen The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay Indonesian Biomedical Journal |
author_facet |
Irene Karlina Rahmi Amtha Boedi Oetomo Roeslan Yuniar Zen |
author_sort |
Irene Karlina |
title |
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay |
title_short |
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay |
title_full |
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay |
title_fullStr |
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay |
title_full_unstemmed |
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay |
title_sort |
release of total metal ion and genotoxicity of stainless steel brackets: experimental study using micronucleus assay |
publisher |
Secretariat of The Indonesian Biomedical Journal |
series |
Indonesian Biomedical Journal |
issn |
2085-3297 2355-9179 |
publishDate |
2016-08-01 |
description |
BACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the biocompatibility of stainless steel brackets, micronucleus assay as one of genotoxicity assay is used in this study. To determine the differences and the correlation of metal ions release and genotoxic activity among three brand stainless steel brackets.
METHODS: Three brands of brackets were immersed in artificial saliva for 672 hours and the release of ion chromium, nikel and iron were examined. The cytokynesis block micronucleus assay (CBMN) using lymphocytes was performed as well.
RESULTS: The highest metal releasing were nickel, cromium, iron, respectively (30.5, 27.2, 23.4 ppb). There was a significant differences between total nickel and iron ion release among three brand brackets (p=0.04, p=0.02).
Genotoxicity of metal ion released was correlated with durration of immersion brackets (p=0.01). Genotoxicity was significant correlated with the release of chromium (p=0.03) and nickel (p=0.01).
CONCLUSION: Genotoxicity of stainless steel brackets was influenced by duration of immersion but not influenced by brand brackets.
KEYWORDS: genotoxicity, stainless steel brackets, metal ion |
url |
http://inabj.org/index.php/ibj/article/view/193 |
work_keys_str_mv |
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