The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay

BACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the b...

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Main Authors: Irene Karlina, Rahmi Amtha, Boedi Oetomo Roeslan, Yuniar Zen
Format: Article
Language:English
Published: Secretariat of The Indonesian Biomedical Journal 2016-08-01
Series:Indonesian Biomedical Journal
Online Access:http://inabj.org/index.php/ibj/article/view/193
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spelling doaj-f09fcd61e9304783a882875352aadc4c2020-11-25T00:18:25ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792016-08-01829710210.18585/inabj.v8i2.193196The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus AssayIrene Karlina0Rahmi Amtha1Boedi Oetomo Roeslan2Yuniar Zen3University of TrisaktiUniversity of TrisaktiUniversity Of TrisaktiUniversity of TrisaktiBACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the biocompatibility of stainless steel brackets, micronucleus assay as one of genotoxicity assay is used in this study. To determine the differences and the correlation of metal ions release and genotoxic activity among three brand stainless steel brackets. METHODS: Three brands of brackets were immersed in artificial saliva for 672 hours and the release of ion chromium, nikel and iron were examined. The cytokynesis block micronucleus assay (CBMN) using lymphocytes was performed as well. RESULTS: The highest metal releasing were nickel, cromium, iron, respectively (30.5, 27.2, 23.4 ppb). There was a significant differences between total nickel and iron ion release among three brand brackets (p=0.04, p=0.02). Genotoxicity of metal ion released was correlated with durration of immersion brackets (p=0.01). Genotoxicity was significant correlated with the release of chromium (p=0.03) and nickel (p=0.01). CONCLUSION: Genotoxicity of stainless steel brackets was influenced by duration of immersion but not influenced by brand brackets.  KEYWORDS: genotoxicity, stainless steel brackets, metal ionhttp://inabj.org/index.php/ibj/article/view/193
collection DOAJ
language English
format Article
sources DOAJ
author Irene Karlina
Rahmi Amtha
Boedi Oetomo Roeslan
Yuniar Zen
spellingShingle Irene Karlina
Rahmi Amtha
Boedi Oetomo Roeslan
Yuniar Zen
The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
Indonesian Biomedical Journal
author_facet Irene Karlina
Rahmi Amtha
Boedi Oetomo Roeslan
Yuniar Zen
author_sort Irene Karlina
title The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
title_short The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
title_full The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
title_fullStr The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
title_full_unstemmed The Release of Total Metal Ion and Genotoxicity of Stainless Steel Brackets: Experimental Study Using Micronucleus Assay
title_sort release of total metal ion and genotoxicity of stainless steel brackets: experimental study using micronucleus assay
publisher Secretariat of The Indonesian Biomedical Journal
series Indonesian Biomedical Journal
issn 2085-3297
2355-9179
publishDate 2016-08-01
description BACKGROUND: Stainless steel brackets are composed of various metal that may corrode in oral cavity. Corrosion is caused by the release of metal ions such as chromium, nickel, and iron. The release of metal ions can cause adverse effects such as toxicity, allergic, and mutagenicity. To evaluate the biocompatibility of stainless steel brackets, micronucleus assay as one of genotoxicity assay is used in this study. To determine the differences and the correlation of metal ions release and genotoxic activity among three brand stainless steel brackets. METHODS: Three brands of brackets were immersed in artificial saliva for 672 hours and the release of ion chromium, nikel and iron were examined. The cytokynesis block micronucleus assay (CBMN) using lymphocytes was performed as well. RESULTS: The highest metal releasing were nickel, cromium, iron, respectively (30.5, 27.2, 23.4 ppb). There was a significant differences between total nickel and iron ion release among three brand brackets (p=0.04, p=0.02). Genotoxicity of metal ion released was correlated with durration of immersion brackets (p=0.01). Genotoxicity was significant correlated with the release of chromium (p=0.03) and nickel (p=0.01). CONCLUSION: Genotoxicity of stainless steel brackets was influenced by duration of immersion but not influenced by brand brackets.  KEYWORDS: genotoxicity, stainless steel brackets, metal ion
url http://inabj.org/index.php/ibj/article/view/193
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