Behavior of spoilage bacteria and Salmonella enterica subspecies enterica O:4,5 in vacuum-packaged beef during refrigeration

• Main Authors: Jorge Luiz da Silva, Vasco Augusto Pilão Cadavez, Maxsueli Aparecida Moura Machado, Brendo da Conceição Lima Dias, Adelino da Cunha-Neto, Ursula Gonzales-Barron, Eduardo Eustáquio de Souza Figueiredo
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria
• Series: Ciência Rural
• Subjects: deteriorating microorganisms; meat quality; predictive microbiology; salmonella
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000700454&lng=en&tlng=en
• ISSN: 0103-8478; 1678-4596

ABSTRACT: In this study, the kinetic parameters of mesophilic, psychrotrophic and lactic acid bacteria in vacuum-packed beef at 1 °C and 4 °C were estimated from experimental growth curves produced by samples stored during 21 and 60 days, respectively. In a separate experiment, the survival of multidrug resistant (MDR) Salmonella enterica O:4,5 at 1°C was also characterized. The shelf-life of vacuum-packed beef stored at 4 °C was estimated at 16.1 days (95% CI: 14.8 - 17.3 days), whereas at 1 °C it was longer than 21 days because the mesophiles count estimated towards the end of the experiment was 12.5 ln CFU.g-1 (95% CI: 11.8 - 13.3 ln CFU.g-1) which is lower than the shelf-life reference value. At 1 °C, inoculated Salmonella was reduced in 6.61 ln CFU.g-1 (2.87 log CFU.g-1). These results demonstrated the importance of establishing in legislation, especially in Brazil, standard values of deteriorating microorganisms in beef for maintaining product quality.