Effects of sleep on the splenic milieu in mice and the T cell receptor repertoire recruited into a T cell dependent B cell response

• Main Authors: Cornelia Tune, Martin Meinhardt, Kathrin Kalies, Rene Pagel, Lisa-Kristin Schierloh, Julia Hahn, Stella E. Autenrieth, Christiane E. Koch, Henrik Oster, Andrea Schampel, Juergen Westermann
• Format: Article
• Language: English
• Published: Elsevier 2020-05-01
• Series: Brain, Behavior, & Immunity - Health
• Subjects: Sleep deprivation; Lymphocyte migration; Antigen presentation; T cell dependent B cell Response; Sheep red blood cells; T cell receptor repertoire
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666354620300478

Sleep is known to improve immune function ranging from cell distribution in the naïve state to elevated antibody titers after an immune challenge. The underlying mechanisms still remain unclear, partially because most studies have focused on the analysis of blood only. Hence, we investigated the effects of sleep within the spleen in female C57BL/6J mice with normal sleep compared to short-term sleep-deprived animals both in the naïve state and after an antigen challenge. Lack of sleep decreased the expression of genes associated with immune cell recruitment into and antigen presentation within the spleen both in the naïve state and during a T cell dependent B cell response directed against sheep red blood cells (SRBC). However, neither T cell proliferation nor formation of SRBC-specific antibodies was affected. In addition, the T cell receptor repertoire recruited into the immune response within seven days was not influenced by sleep deprivation. Thus, sleep modulated the molecular milieu within the spleen whereas we could not detect corresponding changes in the primary immune response against SRBC. Further studies will show whether sleep influences the secondary immune response against SRBC or the development of the B cell receptor repertoire, and how this can be compared to other antigens.