Application of Polyhydroxybutyrate-b-polyethyleneglycol (a block co-polymer) for Solid Phase Extraction of Lead and Copper in Different Food Samples

• Main Authors: Sham K. Wadhwa, Tasneem Gul Kazi, Mustafa Tuzen, Hassan Imran Afridi, Naeemullah, Kapil Dev Brahman
• Format: Article
• Language: English
• Published: National Centre of Excellence in Analytical Chemsitry 2014-06-01
• Series: Pakistan Journal of Analytical & Environmental Chemistry
• Online Access: http://ceacsu.edu.pk/PDF%20file/Volume%2015%20No%201/66-73-PJAEC-27092013-23.pdf

In present work, a new adsorbent, polyhydroxybutyrate-b-polyethyleneglycol (block copolymer) was used for the preconcentration and separation of copper (Cu) and lead (Pb) ions without consuming expensive complexing reagent. The influence of various parameters like pH, adsorbent amount, and rates of flow of eluent, sample and sample volumes has been investigated. The polymer does not interact with alkaline-earth metals, transition metals, alkaline, and few anions. The enrichment factor 50 was achieved in this method. The detection limit of method was found to be 0.36 µg L-1 and 1.93 µg L-1 for copper and lead, respectively. The recovery values of both analytes were found >96% and relative standard deviations (RSD) for all experiments were found less than 5%. The present method was validated by the analysis of Cu and Pb contents in various related certified reference materials (CRM) like; NIST SRM 1515 Apple leaves, IAEA-336 Lichen and GBW-07605 Tea. Found results and CRM values were precise and accurate. This developed method was then successfully applied for analysis of Cu and Pb in tap and bottled mineral water and real food samples.