Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions

Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions

Purpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.

Bibliographic Details
Main Authors: Ruszova E, Chmelarova M, Senkerikova M, Stefackova S
Format: Article
Language:English
Published: Sciendo 2015-12-01
Series:Acta Medica Martiniana
Subjects:
prader willi syndrome
angelman syndrome
methyl-specific semi-quantitative real-time pcr
epigenetics
Online Access:https://doi.org/10.1515/acm-2015-0012
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spelling doaj-f005e5168be84455981898dd963d9b342021-09-06T19:39:37ZengSciendoActa Medica Martiniana 1335-84212015-12-0115351210.1515/acm-2015-0012acm-2015-0012Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted RegionsRuszova E0Chmelarova M1Senkerikova M2Stefackova S3 Department of Clinical Genetics, University Hospital in Hradec KraloveInstitute for Clinical Biochemistry and Diagnostics, University Hospital in Hradec Kralove Department of Clinical Genetics, University Hospital in Hradec KraloveDepartment of Pediatric Neurology, University Hospital in Hradec Kralove, Czech RepublicPurpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.https://doi.org/10.1515/acm-2015-0012prader willi syndromeangelman syndromemethyl-specific semi-quantitative real-time pcrepigenetics
collection DOAJ
language English
format Article
sources DOAJ
author Ruszova E
Chmelarova M
Senkerikova M
Stefackova S
spellingShingle Ruszova E
Chmelarova M
Senkerikova M
Stefackova S
Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
Acta Medica Martiniana
prader willi syndrome
angelman syndrome
methyl-specific semi-quantitative real-time pcr
epigenetics
author_facet Ruszova E
Chmelarova M
Senkerikova M
Stefackova S
author_sort Ruszova E
title Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
title_short Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
title_full Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
title_fullStr Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
title_full_unstemmed Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
title_sort duplex methylation-specific semi-quantitative real-time pcr for cost-effective & time-efficient diagnostic screening of chromosome 15 and 14 imprinted regions
publisher Sciendo
series Acta Medica Martiniana
issn 1335-8421
publishDate 2015-12-01
description Purpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.
topic prader willi syndrome
angelman syndrome
methyl-specific semi-quantitative real-time pcr
epigenetics
url https://doi.org/10.1515/acm-2015-0012
work_keys_str_mv AT ruszovae duplexmethylationspecificsemiquantitativerealtimepcrforcosteffectivetimeefficientdiagnosticscreeningofchromosome15and14imprintedregions
AT chmelarovam duplexmethylationspecificsemiquantitativerealtimepcrforcosteffectivetimeefficientdiagnosticscreeningofchromosome15and14imprintedregions
AT senkerikovam duplexmethylationspecificsemiquantitativerealtimepcrforcosteffectivetimeefficientdiagnosticscreeningofchromosome15and14imprintedregions
AT stefackovas duplexmethylationspecificsemiquantitativerealtimepcrforcosteffectivetimeefficientdiagnosticscreeningofchromosome15and14imprintedregions
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