Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions
Purpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sciendo
2015-12-01
|
Series: | Acta Medica Martiniana |
Subjects: | |
Online Access: | https://doi.org/10.1515/acm-2015-0012 |