Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions

Purpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.

Bibliographic Details
Main Authors: Ruszova E, Chmelarova M, Senkerikova M, Stefackova S
Format: Article
Language:English
Published: Sciendo 2015-12-01
Series:Acta Medica Martiniana
Subjects:
Online Access:https://doi.org/10.1515/acm-2015-0012