Comparison of the frequency and the occurrence of antimicrobial resistance among C. jejuni and C. coli isolated from human infections, retail poultry meat and poultry in Zenica-Doboj Canton, Bosnia and Herzegovina

• Main Authors: Ingrid Berce, Tina Zorman, Selma Uzunović-Kamberović, Lieve Herman, Sonja Smole Možina
• Format: Article
• Language: English
• Published: Medical Association of Zenica-Doboj Canton 2009-08-01
• Series: Medicinski Glasnik
• Subjects: ciprofloxacin-resistance; erythromycin-resistance; Bosnia and Herzegovina; Campylobacter jejuni; Campylobacter coli
• Online Access: http://www.ljkzedo.com.ba/medglasnik/vol62/M10_3.pdf
• ISSN: 1840-0132; 1840-2445

Aim To compare the frequency of isolation and occurrence of antimicrobialresistance among C. jejuni and C. coli isolated in humans,retail poultry meat and poultry.Methods Fifty-three human, 52 retail poultry meat and 15 poultryCampylobacter jejuni/coli isolates were investigated for antibioticsusceptibility to eight antimicrobials by disk-diffusion method.Erythromycin and ciprofloxacin susceptibility were further determinedby E-test, and additionally the MICs of erythromycinand ciprofloxacin were determined using the broth microdilutionmethod.Results Prevalence of C. coli in humans, retail poultry meat andpoultry was 28.3%, 56.9% and 53.3%, respectively. No significantdifferences were found in the overall resistance rates between C.jejuni and C. coli isolated from all three sources (p>0.05). Erythromycinand ciprofloxacin resistance was high and similar in humans,retail poultry meat and poultry (26.4%, 35.3%, 26.7%, and32.1%, 23.5%, 26.7%, respectively) (p>0.05). C. jejuni displayedhigher prevalence of resistance to erythromycin than C. coli inall investigated sources (p>0.05). All ciprofloxacin and 94.4% oferythromycin positive isolates were highly resistant (≥ 32 μg/mLand ≥128 μg/mL, respectively).Conclusion The high prevalence of C. coli isolates from humans,poultry meat and poultry and higher both overall and erythromycin-resistance in C. jejuni than in C. coli isolates suggests that theremay be a common source in the environment, which might be absentin other geographical regions. Further studies are requiredto determine the role of efflux mechanism in erythromycin- andciprofloxacin-resistance related to the level of resistance.