Evaluation of the potential of free and immobilized thermophilic bacterial enzymes in the degradation of agro-industrial wastes
Agro-industrial wastes are potential starting materials for the production of useful value-added compounds, including prebiotic oligosaccharides. In this paper, we evaluated the potential of thermophilic bacterial pectin- and xylan-degrading recombinant enzymes for the degradation of the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400019K.pdf |
| Summary: | Agro-industrial wastes are potential starting materials for the production of
useful value-added compounds, including prebiotic oligosaccharides. In this
paper, we evaluated the potential of thermophilic bacterial pectin- and
xylan-degrading recombinant enzymes for the degradation of the
agro-industrial wastes: apple pomace, wheat straw, wheat bran and distillers
grains. For the immobilization of pectate lyase and xylanase, three different
supports were used. The effect of enzyme immobilization was analyzed in terms
of enhanced thermostability and activity against these wastes. For xylanase,
the highest thermostability was achieved by immobilization on Sepabeads
EC-EP/M. The best activity against bran and grains was obtained by
immobilization on Sepabeads EC-HA/M. For pectate lyase, the highest
thermostability was achieved by immobilization on Sepabeads EC-EP/M, however,
activity against apple pomace pectin was slightly reduced by this
immobilization. The length of oligosaccharides produced by both free and
immobilized enzymes was also determined. |
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| ISSN: | 0354-4664 1821-4339 |