Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine...

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Main Authors: Priscilla Barbosa Ferreira SOARES, Aletheia Moraes ROCHA, Manuella Verdinelli de Paula REIS, Camilla Christian Gomes MOURA, Carlos José SOARES
Format: Article
Language:English
Published: Universidade Estadual Paulista
Series:Revista de Odontologia da UNESP
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234&lng=en&tlng=en
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spelling doaj-efc52ebd44194e0b9cd83b0c97ee1b5e2020-11-24T22:53:49ZengUniversidade Estadual PaulistaRevista de Odontologia da UNESP1807-257745423423910.1590/1807-2577.09216S1807-25772016000400234Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro studyPriscilla Barbosa Ferreira SOARESAletheia Moraes ROCHAManuella Verdinelli de Paula REISCamilla Christian Gomes MOURACarlos José SOARESAbstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234&lng=en&tlng=enTooth avulsioncoconut watercell viability
collection DOAJ
language English
format Article
sources DOAJ
author Priscilla Barbosa Ferreira SOARES
Aletheia Moraes ROCHA
Manuella Verdinelli de Paula REIS
Camilla Christian Gomes MOURA
Carlos José SOARES
spellingShingle Priscilla Barbosa Ferreira SOARES
Aletheia Moraes ROCHA
Manuella Verdinelli de Paula REIS
Camilla Christian Gomes MOURA
Carlos José SOARES
Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
Revista de Odontologia da UNESP
Tooth avulsion
coconut water
cell viability
author_facet Priscilla Barbosa Ferreira SOARES
Aletheia Moraes ROCHA
Manuella Verdinelli de Paula REIS
Camilla Christian Gomes MOURA
Carlos José SOARES
author_sort Priscilla Barbosa Ferreira SOARES
title Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_short Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_full Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_fullStr Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_full_unstemmed Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_sort evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
publisher Universidade Estadual Paulista
series Revista de Odontologia da UNESP
issn 1807-2577
description Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.
topic Tooth avulsion
coconut water
cell viability
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234&lng=en&tlng=en
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