Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota
A single-band protein (HEP3) was isolated from Hericium erinaceus using a chemical separation combined with pharmacodynamic evaluation methods. This protein exhibited immunomodulatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by decreasing the overproduction of tumor necrosis fa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.00666/full |
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doaj-efa95237a9a84dee95446d92385e8bfc |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Chen Diling Chen Diling Chen Diling Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Yang Jian Yang Jian Yang Jian Li Jian Li Jian Li Jian Li Jian Su Jiyan Su Jiyan Su Jiyan Xie Yizhen Xie Yizhen Xie Yizhen Xie Yizhen Lai Guoxiao Lai Guoxiao Lai Guoxiao Lai Guoxiao |
| spellingShingle |
Chen Diling Chen Diling Chen Diling Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Yang Jian Yang Jian Yang Jian Li Jian Li Jian Li Jian Li Jian Su Jiyan Su Jiyan Su Jiyan Xie Yizhen Xie Yizhen Xie Yizhen Xie Yizhen Lai Guoxiao Lai Guoxiao Lai Guoxiao Lai Guoxiao Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota Frontiers in Immunology anti-inflammation functional food ingredient fungal immunomodulatory protein gut microbiota Hericium erinaceus immunotherapy |
| author_facet |
Chen Diling Chen Diling Chen Diling Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Zheng Chaoqun Yang Jian Yang Jian Yang Jian Li Jian Li Jian Li Jian Li Jian Su Jiyan Su Jiyan Su Jiyan Xie Yizhen Xie Yizhen Xie Yizhen Xie Yizhen Lai Guoxiao Lai Guoxiao Lai Guoxiao Lai Guoxiao |
| author_sort |
Chen Diling |
| title |
Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota |
| title_short |
Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota |
| title_full |
Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota |
| title_fullStr |
Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota |
| title_full_unstemmed |
Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota |
| title_sort |
immunomodulatory activities of a fungal protein extracted from hericium erinaceus through regulating the gut microbiota |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Immunology |
| issn |
1664-3224 |
| publishDate |
2017-06-01 |
| description |
A single-band protein (HEP3) was isolated from Hericium erinaceus using a chemical separation combined with pharmacodynamic evaluation methods. This protein exhibited immunomodulatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by decreasing the overproduction of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, and downregulating the expression of inducible nitric oxide synthase and nuclear factor-κB p65. Further researches revealed that HEP3 could improve the immune system via regulating the composition and metabolism of gut microbiota to activate the proliferation and differentiation of T cells, stimulate the intestinal antigen-presenting cells in high-dose cyclophosphamide-induced immunotoxicity in mice, and play a prebiotic role in the case of excessive antibiotics in inflammatory bowel disease model mice. Aided experiments also showed that HEP3 could be used as an antitumor immune inhibitor in tumor-burdened mice. The results of the present study suggested that fungal protein from H. erinaceus could be used as a drug or functional food ingredient for immunotherapy because of its immunomodulatory activities. |
| topic |
anti-inflammation functional food ingredient fungal immunomodulatory protein gut microbiota Hericium erinaceus immunotherapy |
| url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.00666/full |
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doaj-efa95237a9a84dee95446d92385e8bfc2020-11-24T21:46:02ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-06-01810.3389/fimmu.2017.00666243034Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut MicrobiotaChen Diling0Chen Diling1Chen Diling2Zheng Chaoqun3Zheng Chaoqun4Zheng Chaoqun5Zheng Chaoqun6Yang Jian7Yang Jian8Yang Jian9Li Jian10Li Jian11Li Jian12Li Jian13Su Jiyan14Su Jiyan15Su Jiyan16Xie Yizhen17Xie Yizhen18Xie Yizhen19Xie Yizhen20Lai Guoxiao21Lai Guoxiao22Lai Guoxiao23Lai Guoxiao24State Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaCollege of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaCollege of Chinese Materia Medica, Guangxi University of Traditional Chinese Medicine, Nanning, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou, ChinaState Key Laboratory of Applied Microbiology South China, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, ChinaCollege of Chinese Materia Medica, Guangxi University of Traditional Chinese Medicine, Nanning, ChinaA single-band protein (HEP3) was isolated from Hericium erinaceus using a chemical separation combined with pharmacodynamic evaluation methods. This protein exhibited immunomodulatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by decreasing the overproduction of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, and downregulating the expression of inducible nitric oxide synthase and nuclear factor-κB p65. Further researches revealed that HEP3 could improve the immune system via regulating the composition and metabolism of gut microbiota to activate the proliferation and differentiation of T cells, stimulate the intestinal antigen-presenting cells in high-dose cyclophosphamide-induced immunotoxicity in mice, and play a prebiotic role in the case of excessive antibiotics in inflammatory bowel disease model mice. Aided experiments also showed that HEP3 could be used as an antitumor immune inhibitor in tumor-burdened mice. The results of the present study suggested that fungal protein from H. erinaceus could be used as a drug or functional food ingredient for immunotherapy because of its immunomodulatory activities.http://journal.frontiersin.org/article/10.3389/fimmu.2017.00666/fullanti-inflammationfunctional food ingredientfungal immunomodulatory proteingut microbiotaHericium erinaceusimmunotherapy |