In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).

It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture...

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Main Authors: Xin Hu, Jiang-Hui Li, Lan Lan, Fei-Fei Wu, Er-Peng Zhang, Zeng-Mei Song, Hui-Cong Huang, Fang-Jun Luo, Chang-Wang Pan, Feng Tan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3290544?pdf=render
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spelling doaj-ef6e5db5504e4575bc2309082d0e49762020-11-25T00:10:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3216110.1371/journal.pone.0032161In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).Xin HuJiang-Hui LiLan LanFei-Fei WuEr-Peng ZhangZeng-Mei SongHui-Cong HuangFang-Jun LuoChang-Wang PanFeng TanIt has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture brain microvascular endothelial cells (BMECs) and brain astrocytic cells (BACs). In the present study, a four-hour percolation and HRP permeability experiment showed that A. cantonensis larvae extracts can increase the permeability of the BBB. Apoptosis among BMECs and BACs after exposure to larvae extracts was monitored by TUNEL and annexin-V-FITC/PI double staining. A. cantonensis larvae extracts were found to induce apoptosis in both BMECs and BACs. For this reason, we concluded that the induction of apoptosis might participate in the BBB dysfunction observed during angiostrongyliasis. Improved fundamental understanding of how A. cantonensis induces apoptosis may lead to new approaches to the treatment or prevention of this parasitic disease.http://europepmc.org/articles/PMC3290544?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Xin Hu
Jiang-Hui Li
Lan Lan
Fei-Fei Wu
Er-Peng Zhang
Zeng-Mei Song
Hui-Cong Huang
Fang-Jun Luo
Chang-Wang Pan
Feng Tan
spellingShingle Xin Hu
Jiang-Hui Li
Lan Lan
Fei-Fei Wu
Er-Peng Zhang
Zeng-Mei Song
Hui-Cong Huang
Fang-Jun Luo
Chang-Wang Pan
Feng Tan
In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
PLoS ONE
author_facet Xin Hu
Jiang-Hui Li
Lan Lan
Fei-Fei Wu
Er-Peng Zhang
Zeng-Mei Song
Hui-Cong Huang
Fang-Jun Luo
Chang-Wang Pan
Feng Tan
author_sort Xin Hu
title In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
title_short In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
title_full In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
title_fullStr In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
title_full_unstemmed In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
title_sort in vitro study of the effects of angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (bbb).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture brain microvascular endothelial cells (BMECs) and brain astrocytic cells (BACs). In the present study, a four-hour percolation and HRP permeability experiment showed that A. cantonensis larvae extracts can increase the permeability of the BBB. Apoptosis among BMECs and BACs after exposure to larvae extracts was monitored by TUNEL and annexin-V-FITC/PI double staining. A. cantonensis larvae extracts were found to induce apoptosis in both BMECs and BACs. For this reason, we concluded that the induction of apoptosis might participate in the BBB dysfunction observed during angiostrongyliasis. Improved fundamental understanding of how A. cantonensis induces apoptosis may lead to new approaches to the treatment or prevention of this parasitic disease.
url http://europepmc.org/articles/PMC3290544?pdf=render
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