Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus

In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag...

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Main Authors:	Y Sogame, K Kojima, T Takeshita, E Kinoshita, T Matsuoka
Format:	Article
Language:	English
Published:	University of Modena and Reggio Emilia 2014-06-01
Series:	Invertebrate Survival Journal
Subjects:	environment-resistant cyst Colpoda encystment protein phosphorylation cAMP
Online Access:	https://isj02.unimore.it/index.php/ISJ/article/view/323

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spelling	doaj-ef6460ae411947eb8b3ed137aa46b8b72020-12-02T18:27:01ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2014-06-01111Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullusY Sogame0K Kojima1T Takeshita2E Kinoshita3T Matsuoka4Department of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, JapanDepartment of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanDepartment of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanDepartment of Functional Molecular Science, Institute of Biomedical & Health Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8553, JapanDepartment of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, Japan In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag agarose phosphate-affinity beads and subsequent SDS-PAGE. In a liquid chromatography/tandem mass spectrometry analysis of these phosphoproteins, 27-, 37- and 43-kDa proteins (p27, p37 and p43) were identified as Rieske iron-sulfur protein, histone H4 (hyperacetylated form), and actin, respectively. https://isj02.unimore.it/index.php/ISJ/article/view/323environment-resistant cystColpodaencystmentprotein phosphorylationcAMP
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka
spellingShingle	Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus Invertebrate Survival Journal environment-resistant cyst Colpoda encystment protein phosphorylation cAMP
author_facet	Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka
author_sort	Y Sogame
title	Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
title_short	Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
title_full	Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
title_fullStr	Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
title_full_unstemmed	Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
title_sort	identification of camp-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate colpoda cucullus
publisher	University of Modena and Reggio Emilia
series	Invertebrate Survival Journal
issn	1824-307X
publishDate	2014-06-01
description	In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag agarose phosphate-affinity beads and subsequent SDS-PAGE. In a liquid chromatography/tandem mass spectrometry analysis of these phosphoproteins, 27-, 37- and 43-kDa proteins (p27, p37 and p43) were identified as Rieske iron-sulfur protein, histone H4 (hyperacetylated form), and actin, respectively.
topic	environment-resistant cyst Colpoda encystment protein phosphorylation cAMP
url	https://isj02.unimore.it/index.php/ISJ/article/view/323
work_keys_str_mv	AT ysogame identificationofcampdependentphosphorylatedproteinsinvolvedintheformationofenvironmentresistantrestingcystsbytheterrestrialciliatecolpodacucullus AT kkojima identificationofcampdependentphosphorylatedproteinsinvolvedintheformationofenvironmentresistantrestingcystsbytheterrestrialciliatecolpodacucullus AT ttakeshita identificationofcampdependentphosphorylatedproteinsinvolvedintheformationofenvironmentresistantrestingcystsbytheterrestrialciliatecolpodacucullus AT ekinoshita identificationofcampdependentphosphorylatedproteinsinvolvedintheformationofenvironmentresistantrestingcystsbytheterrestrialciliatecolpodacucullus AT tmatsuoka identificationofcampdependentphosphorylatedproteinsinvolvedintheformationofenvironmentresistantrestingcystsbytheterrestrialciliatecolpodacucullus
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Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus

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