| Summary: | Lignocellulose comprised of cellulose and hemicellulose is one of the most abundant renewable feedstocks. Lactic acid bacteria have the ability to ferment sugar derived from lignocellulose. In this study, <i>Pediococcus acidilactici</i> PA204 is a lactic acid bacterium with a high tolerance of temperature and high-efficiency utilization of xylose. We developed a fed-batch simultaneous saccharification and fermentation (SSF) process at 37 °C (pH 6.0) using the 30 FPU (filter paper units)/g cellulase and 20 g/L corn steep powder in a 5 L bioreactor to produce lactic acid (LA). The titer, yield, and productivity of LA produced from 12% (<i>w</i>/<i>w</i>) NaOH-pretreated and washed stover were 92.01 g/L, 0.77 g/g stover, and 1.28 g/L/h, respectively, and those from 15% NaOH-pretreated and washed stover were 104.11 g/L, 0.69 g/g stover, and 1.24 g/L/h, respectively. This study develops a feasible fed-batch SSF process for LA production from corn stover and provides a promising candidate strain for high-titer and -yield lignocellulose-derived LA production.
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