Comparing the Effects of Benzyladenine and <i>meta</i>-Topolin on Sweet Basil (<i>Ocimum basilicum</i>) Micropropagation
<p align="justify"> <span lang="EN-GB" style="font-size: 10.0pt; font-family: 'Times New Roman',serif; letter-spacing: -.2pt">Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of unifo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
2014-12-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/9464 |
| Summary: | <p align="justify">
<span lang="EN-GB" style="font-size: 10.0pt; font-family: 'Times New Roman',serif; letter-spacing: -.2pt">Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of uniform quality. The application of <i>meta</i>-Topolin (<i>m</i>T) (N<sup>6</sup>-(2-hydroxybenzyl) adenine-9-riboside) and aromatic cytokinin as Benzyladenine (BAP) in the micro propagation of sweet basil (<i>Ocimum basilicum </i>L.) was tested for the first time and plant growth parameters assessed to determine the optimum level of these cytokinins. Additionally, the rate of root-growth inhibition due to these two cytokinins was also assessed. Our results show that 1 mg/l (4.43 </span><span lang="EN-GB" style="font-size: 10.0pt; font-family: Symbol; letter-spacing: -.2pt">m</span><span lang="EN-GB" style="font-size: 10.0pt; font-family: 'Times New Roman',serif; letter-spacing: -.2pt">M) BAP and 0.5 mg/l (2.07 </span><span lang="EN-GB" style="font-size: 10.0pt; font-family: Symbol; letter-spacing: -.2pt">m</span><span lang="EN-GB" style="font-size: 10.0pt; font-family: 'Times New Roman',serif; letter-spacing: -.2pt">M)<i>m</i>T produced the most favourable effects on new shoot developments. <i>Meta</i>-Topolin was shown to increase the quality of the plants and in comparison with BAP fewer distortions were observed. No significant differences in root-growth inhibition between the <i>m</i>T and BAP were detected.</span><span style="font-size: 10.0pt; font-family: 'Times New Roman',serif; letter-spacing: -.2pt">
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| ISSN: | 2067-3205 2067-3264 |