Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA

Nasal swaps were collected from 405 elementary school boys of eight governmental schools located within several districts in Al-Madinah city to be screened for asymptomatic carriage of potential Staphylococcus pathogens. A total of 138 Gram-positive staphylococci isolates (34%) showing blood hemolys...

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Main Authors: Sultan M. Alsharif, Wael S. El-Sayed, Ahmed M. Hanafy
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:Journal of Taibah University for Science
Subjects:
Online Access:http://dx.doi.org/10.1080/16583655.2021.1892991
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spelling doaj-eef93b3ebcdf42099a5a2a879ba513012021-04-06T13:27:31ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552021-01-0115112213210.1080/16583655.2021.18929911892991Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSASultan M. Alsharif0Wael S. El-Sayed1Ahmed M. Hanafy2Taibah UniversityAin Shams UniversityTaibah UniversityNasal swaps were collected from 405 elementary school boys of eight governmental schools located within several districts in Al-Madinah city to be screened for asymptomatic carriage of potential Staphylococcus pathogens. A total of 138 Gram-positive staphylococci isolates (34%) showing blood hemolysis were selected. Based on their morphological, cultural characteristics and Restriction enzyme analysis Isolates were grouped into 12 phenotypic within four distinct clusters. The 16S rDNA sequence analysis of all isolates revealed S. aureus to be the most prevalent (80%), followed by S. epidermidis (9%) and finally S. hominis and S. simulans accounting for 6% and 5% respectively. Staphylococcus isolates were detected with higher frequency in participants of age group 7–9 years, with the lowest numbers of staphylococci (18%) isolated from the schools located in the western area. Multiplex PCR assay revealed that all S. aureus isolates (20%) which were phenotypically recognized as MRSA by the Kirby–Bauer disc diffusion method contained the mecA gene. MecA gene was also detected in three coagulase-negative staphylococci isolates.http://dx.doi.org/10.1080/16583655.2021.1892991staphylococcusschool boysasymptomatic carriagemrsamultiplex pcr
collection DOAJ
language English
format Article
sources DOAJ
author Sultan M. Alsharif
Wael S. El-Sayed
Ahmed M. Hanafy
spellingShingle Sultan M. Alsharif
Wael S. El-Sayed
Ahmed M. Hanafy
Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
Journal of Taibah University for Science
staphylococcus
school boys
asymptomatic carriage
mrsa
multiplex pcr
author_facet Sultan M. Alsharif
Wael S. El-Sayed
Ahmed M. Hanafy
author_sort Sultan M. Alsharif
title Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
title_short Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
title_full Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
title_fullStr Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
title_full_unstemmed Geographic distribution and prevalence of potential asymptomatic Staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at Al-Madinah, KSA
title_sort geographic distribution and prevalence of potential asymptomatic staphylococcus spp. in the nasopharyngeal cavity of elementary school boys at al-madinah, ksa
publisher Taylor & Francis Group
series Journal of Taibah University for Science
issn 1658-3655
publishDate 2021-01-01
description Nasal swaps were collected from 405 elementary school boys of eight governmental schools located within several districts in Al-Madinah city to be screened for asymptomatic carriage of potential Staphylococcus pathogens. A total of 138 Gram-positive staphylococci isolates (34%) showing blood hemolysis were selected. Based on their morphological, cultural characteristics and Restriction enzyme analysis Isolates were grouped into 12 phenotypic within four distinct clusters. The 16S rDNA sequence analysis of all isolates revealed S. aureus to be the most prevalent (80%), followed by S. epidermidis (9%) and finally S. hominis and S. simulans accounting for 6% and 5% respectively. Staphylococcus isolates were detected with higher frequency in participants of age group 7–9 years, with the lowest numbers of staphylococci (18%) isolated from the schools located in the western area. Multiplex PCR assay revealed that all S. aureus isolates (20%) which were phenotypically recognized as MRSA by the Kirby–Bauer disc diffusion method contained the mecA gene. MecA gene was also detected in three coagulase-negative staphylococci isolates.
topic staphylococcus
school boys
asymptomatic carriage
mrsa
multiplex pcr
url http://dx.doi.org/10.1080/16583655.2021.1892991
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