Construction and characterization of the rat neutral amino acid transporter HA － SNAT3 fusion protein
Sodium-coupled neutral amino acid transporter 3 (SNAT3) is a member of SLC38 gene family which primarily expresses in astrocytes in the brain and retina.It transports L-glutamine predominantly and plays an important role in glutamate-glutamine cycle in the brain and liver.In order to easily detect t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals Center of Shanghai Normal University
2014-06-01
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| Series: | Journal of Shanghai Normal University (Natural Sciences) |
| Subjects: | |
| Online Access: | http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/create_pdf.aspx?file_no=201403001&flag=1&year_id=2014&quarter_id=3 |
| Summary: | Sodium-coupled neutral amino acid transporter 3 (SNAT3) is a member of SLC38 gene family which primarily expresses in astrocytes in the brain and retina.It transports L-glutamine predominantly and plays an important role in glutamate-glutamine cycle in the brain and liver.In order to easily detect the expression and localization of SNAT3 on the membrane,we constructed a eukaryotic expression plasmid with an HA tag at the N-terminus of rat SNAT3 in the mammalian expression vector p<i>BK-CMVΔ(1098-1300)</i> by PCR and double restriction endonuclease digestion techniques.After transiently transfected the plasmid into human embryonic kidney cells (HEK293T),expression of HA-SNAT3 fusion protein was detected by western boltting.The result suggested that the HA-SNAT3 fusion protein could normally express on the membrane.The plasmid pBK-CMVΔ(1098-1300)-HA-SNAT3 will provide a useful tool for the investigation of structure and function of SNAT3 in the future. |
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| ISSN: | 1000-5137 1000-5137 |