Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
Introduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although t...
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doaj-eed42cb99c4541b3b850dc22a1ec8bfc2020-11-24T23:04:30ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572017-03-0137013514210.7705/biomedica.v37i0.34742080Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in ColombiaAndrés Gómez-Palacio0Juan Suaza-Vasco1Sandra Castaño2Omar Triana3Sandra Uribe4Grupo de Investigación en Ciencias Biomédicas, Escuela de Ciencias Biológicas, Universidad Pedagógica y Tecnológica de Colombia, Tunja, ColombiaGrupo de Sistemática Molecular, Universidad Nacional de Colombia, Medellín, Colombia Programa de Estudio y Control de Enfermedades Tropicales, Universidad de Antioquia, Medellín, ColombiaGrupo de Biología y Control de Enfermedades Infecciosas, Instituto de Biología, Universidad de Antioquia, Medellín, ColombiaGrupo de Biología y Control de Enfermedades Infecciosas, Instituto de Biología, Universidad de Antioquia, Medellín, ColombiaGrupo de Sistemática Molecular, Universidad Nacional de Colombia, Medellín, ColombiaIntroduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known. Objective: To identify the presence of Ae. albopictus specimens naturally infected with dengue virus collected in Medellín. Materials and methods: Insects were collected in the Universidad Nacional de Colombia campus in Medellín. Individuals were classified as Ae. albopictus and confirmed by DNA barcode region analysis. Mosquitoes were processed for dengue virus identification, and a fragment of the NS3 gen was sequenced and compared with DENV-2 genotypes reported in the literature. Results: Sequence analysis of COI indicated Ae. albopictus individuals were similar to those recently reported in Colombia, and genetically close to those from other regions worldwide. Among the pools tested one was positive for DENV-2, and the NS3 analysis indicated it belonged to the Asian-American clade. Conclusion: We report the presence Ae. albopictus naturally infected with the Asian-American genotype of DENV-2 in Colombia. The presence of Ae. albopictus specimens carrying the most common genotype infecting humans in a highly populated city such as Medellín indicates its potential role as dengue vector in Colombia and highlights the relevance of including it in current vector surveillance strategies.https://www.revistabiomedica.org/index.php/biomedica/article/view/3474Aedesvector controldengue virusgenotypephylogenyColombia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrés Gómez-Palacio Juan Suaza-Vasco Sandra Castaño Omar Triana Sandra Uribe |
spellingShingle |
Andrés Gómez-Palacio Juan Suaza-Vasco Sandra Castaño Omar Triana Sandra Uribe Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia Biomédica: revista del Instituto Nacional de Salud Aedes vector control dengue virus genotype phylogeny Colombia |
author_facet |
Andrés Gómez-Palacio Juan Suaza-Vasco Sandra Castaño Omar Triana Sandra Uribe |
author_sort |
Andrés Gómez-Palacio |
title |
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia |
title_short |
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia |
title_full |
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia |
title_fullStr |
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia |
title_full_unstemmed |
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia |
title_sort |
aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia |
publisher |
Instituto Nacional de Salud |
series |
Biomédica: revista del Instituto Nacional de Salud |
issn |
0120-4157 0120-4157 |
publishDate |
2017-03-01 |
description |
Introduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known.
Objective: To identify the presence of Ae. albopictus specimens naturally infected with dengue virus collected in Medellín.
Materials and methods: Insects were collected in the Universidad Nacional de Colombia campus in Medellín. Individuals were classified as Ae. albopictus and confirmed by DNA barcode region analysis. Mosquitoes were processed for dengue virus identification, and a fragment of the NS3 gen was sequenced and compared with DENV-2 genotypes reported in the literature.
Results: Sequence analysis of COI indicated Ae. albopictus individuals were similar to those recently reported in Colombia, and genetically close to those from other regions worldwide. Among the pools tested one was positive for DENV-2, and the NS3 analysis indicated it belonged to the Asian-American clade.
Conclusion: We report the presence Ae. albopictus naturally infected with the Asian-American genotype of DENV-2 in Colombia. The presence of Ae. albopictus specimens carrying the most common genotype infecting humans in a highly populated city such as Medellín indicates its potential role as dengue vector in Colombia and highlights the relevance of including it in current vector surveillance strategies. |
topic |
Aedes vector control dengue virus genotype phylogeny Colombia |
url |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3474 |
work_keys_str_mv |
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