Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia

Introduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although t...

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Main Authors: Andrés Gómez-Palacio, Juan Suaza-Vasco, Sandra Castaño, Omar Triana, Sandra Uribe
Format: Article
Language:English
Published: Instituto Nacional de Salud 2017-03-01
Series:Biomédica: revista del Instituto Nacional de Salud
Subjects:
Online Access:https://www.revistabiomedica.org/index.php/biomedica/article/view/3474
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spelling doaj-eed42cb99c4541b3b850dc22a1ec8bfc2020-11-24T23:04:30ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572017-03-0137013514210.7705/biomedica.v37i0.34742080Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in ColombiaAndrés Gómez-Palacio0Juan Suaza-Vasco1Sandra Castaño2Omar Triana3Sandra Uribe4Grupo de Investigación en Ciencias Biomédicas, Escuela de Ciencias Biológicas, Universidad Pedagógica y Tecnológica de Colombia, Tunja, ColombiaGrupo de Sistemática Molecular, Universidad Nacional de Colombia, Medellín, Colombia Programa de Estudio y Control de Enfermedades Tropicales, Universidad de Antioquia, Medellín, ColombiaGrupo de Biología y Control de Enfermedades Infecciosas, Instituto de Biología, Universidad de Antioquia, Medellín, ColombiaGrupo de Biología y Control de Enfermedades Infecciosas, Instituto de Biología, Universidad de Antioquia, Medellín, ColombiaGrupo de Sistemática Molecular, Universidad Nacional de Colombia, Medellín, ColombiaIntroduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known. Objective: To identify the presence of Ae. albopictus specimens naturally infected with dengue virus collected in Medellín. Materials and methods: Insects were collected in the Universidad Nacional de Colombia campus in Medellín. Individuals were classified as Ae. albopictus and confirmed by DNA barcode region analysis. Mosquitoes were processed for dengue virus identification, and a fragment of the NS3 gen was sequenced and compared with DENV-2 genotypes reported in the literature. Results: Sequence analysis of COI indicated Ae. albopictus individuals were similar to those recently reported in Colombia, and genetically close to those from other regions worldwide. Among the pools tested one was positive for DENV-2, and the NS3 analysis indicated it belonged to the Asian-American clade. Conclusion: We report the presence Ae. albopictus naturally infected with the Asian-American genotype of DENV-2 in Colombia. The presence of Ae. albopictus specimens carrying the most common genotype infecting humans in a highly populated city such as Medellín indicates its potential role as dengue vector in Colombia and highlights the relevance of including it in current vector surveillance strategies.https://www.revistabiomedica.org/index.php/biomedica/article/view/3474Aedesvector controldengue virusgenotypephylogenyColombia
collection DOAJ
language English
format Article
sources DOAJ
author Andrés Gómez-Palacio
Juan Suaza-Vasco
Sandra Castaño
Omar Triana
Sandra Uribe
spellingShingle Andrés Gómez-Palacio
Juan Suaza-Vasco
Sandra Castaño
Omar Triana
Sandra Uribe
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
Biomédica: revista del Instituto Nacional de Salud
Aedes
vector control
dengue virus
genotype
phylogeny
Colombia
author_facet Andrés Gómez-Palacio
Juan Suaza-Vasco
Sandra Castaño
Omar Triana
Sandra Uribe
author_sort Andrés Gómez-Palacio
title Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
title_short Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
title_full Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
title_fullStr Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
title_full_unstemmed Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
title_sort aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia
publisher Instituto Nacional de Salud
series Biomédica: revista del Instituto Nacional de Salud
issn 0120-4157
0120-4157
publishDate 2017-03-01
description Introduction: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known. Objective: To identify the presence of Ae. albopictus specimens naturally infected with dengue virus collected in Medellín. Materials and methods: Insects were collected in the Universidad Nacional de Colombia campus in Medellín. Individuals were classified as Ae. albopictus and confirmed by DNA barcode region analysis. Mosquitoes were processed for dengue virus identification, and a fragment of the NS3 gen was sequenced and compared with DENV-2 genotypes reported in the literature. Results: Sequence analysis of COI indicated Ae. albopictus individuals were similar to those recently reported in Colombia, and genetically close to those from other regions worldwide. Among the pools tested one was positive for DENV-2, and the NS3 analysis indicated it belonged to the Asian-American clade. Conclusion: We report the presence Ae. albopictus naturally infected with the Asian-American genotype of DENV-2 in Colombia. The presence of Ae. albopictus specimens carrying the most common genotype infecting humans in a highly populated city such as Medellín indicates its potential role as dengue vector in Colombia and highlights the relevance of including it in current vector surveillance strategies.
topic Aedes
vector control
dengue virus
genotype
phylogeny
Colombia
url https://www.revistabiomedica.org/index.php/biomedica/article/view/3474
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