Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation

Background. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concen...

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Main Authors: Kristin Mai, Andreas Boldt, Hans-Michael Hau, Michael Kirschfink, Stephan Schiekofer, Frieder Keller, Joachim Beige, Athanassios Giannis, Ulrich Sack, Franz Maximilian Rasche
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2019/8389765
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spelling doaj-eec42cc18fd44574a3b558e4736cd8912021-07-02T08:54:45ZengHindawi LimitedAnalytical Cellular Pathology2210-71772210-71852019-01-01201910.1155/2019/83897658389765Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal TransplantationKristin Mai0Andreas Boldt1Hans-Michael Hau2Michael Kirschfink3Stephan Schiekofer4Frieder Keller5Joachim Beige6Athanassios Giannis7Ulrich Sack8Franz Maximilian Rasche9Institute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyInstitute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyDepartment of Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Leipzig, Leipzig, GermanyInstitute of Immunology, University of Heidelberg, Heidelberg, GermanyCenter for Geriatric Medicine, Bezirksklinikum Regensburg, Regensburg, GermanyMedical Department I, Nephrology Division, University Hospital Ulm, Ulm, GermanyDepartment of Nephrology, KfH Renal Unit, Hospital St. Georg, Leipzig, GermanyInstitute for Organic Chemistry, University of Leipzig, Leipzig, GermanyInstitute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyDepartment of Internal Medicine, Neurology and Dermatology, Clinic for Endocrinology and Nephrology, Section of Nephrology, University Hospital Leipzig, Leipzig, GermanyBackground. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concentrations of TGFβ, sCD30 (ELISA), and interleukins (CBA) of fifteen patients eligible for renal transplantation were analyzed before, during, and after a regular HD. Results. Before HD, the median proportion of CD8+ effector cells, CD8+ CCR5+ effector cells, and HLA-DR+ regulatory T cells as well as the median concentration of soluble CD30 increased and naive CD8+ T cells decreased. During HD, there was a significant decrease in CD4- CD8- T cells (p<0.001) and an increase in CD25+ T cells (p=0.026), sCD30 (p<0.001), HLA-DR+ regulatory T cells (p=0.005), and regulatory T cells (p=0.003). TGFβ and sCD30 increased significantly over time. The activity of the classical complement pathway started to slightly increase after the first hour of HD and lasted until fifteen minutes after finishing dialysis. The decrease in the functional activity of the alternative pathway was only transient and was followed by a significant increase within 15 minutes after finishing the treatment. Conclusion. HD might interact with the allograft outcome by influencing T cell subsets and activation of the complement system in a biphasic course.http://dx.doi.org/10.1155/2019/8389765
collection DOAJ
language English
format Article
sources DOAJ
author Kristin Mai
Andreas Boldt
Hans-Michael Hau
Michael Kirschfink
Stephan Schiekofer
Frieder Keller
Joachim Beige
Athanassios Giannis
Ulrich Sack
Franz Maximilian Rasche
spellingShingle Kristin Mai
Andreas Boldt
Hans-Michael Hau
Michael Kirschfink
Stephan Schiekofer
Frieder Keller
Joachim Beige
Athanassios Giannis
Ulrich Sack
Franz Maximilian Rasche
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
Analytical Cellular Pathology
author_facet Kristin Mai
Andreas Boldt
Hans-Michael Hau
Michael Kirschfink
Stephan Schiekofer
Frieder Keller
Joachim Beige
Athanassios Giannis
Ulrich Sack
Franz Maximilian Rasche
author_sort Kristin Mai
title Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
title_short Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
title_full Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
title_fullStr Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
title_full_unstemmed Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
title_sort immunological alterations due to hemodialysis might interfere with early complications in renal transplantation
publisher Hindawi Limited
series Analytical Cellular Pathology
issn 2210-7177
2210-7185
publishDate 2019-01-01
description Background. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concentrations of TGFβ, sCD30 (ELISA), and interleukins (CBA) of fifteen patients eligible for renal transplantation were analyzed before, during, and after a regular HD. Results. Before HD, the median proportion of CD8+ effector cells, CD8+ CCR5+ effector cells, and HLA-DR+ regulatory T cells as well as the median concentration of soluble CD30 increased and naive CD8+ T cells decreased. During HD, there was a significant decrease in CD4- CD8- T cells (p<0.001) and an increase in CD25+ T cells (p=0.026), sCD30 (p<0.001), HLA-DR+ regulatory T cells (p=0.005), and regulatory T cells (p=0.003). TGFβ and sCD30 increased significantly over time. The activity of the classical complement pathway started to slightly increase after the first hour of HD and lasted until fifteen minutes after finishing dialysis. The decrease in the functional activity of the alternative pathway was only transient and was followed by a significant increase within 15 minutes after finishing the treatment. Conclusion. HD might interact with the allograft outcome by influencing T cell subsets and activation of the complement system in a biphasic course.
url http://dx.doi.org/10.1155/2019/8389765
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