Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
Background. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concen...
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Series: | Analytical Cellular Pathology |
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doaj-eec42cc18fd44574a3b558e4736cd8912021-07-02T08:54:45ZengHindawi LimitedAnalytical Cellular Pathology2210-71772210-71852019-01-01201910.1155/2019/83897658389765Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal TransplantationKristin Mai0Andreas Boldt1Hans-Michael Hau2Michael Kirschfink3Stephan Schiekofer4Frieder Keller5Joachim Beige6Athanassios Giannis7Ulrich Sack8Franz Maximilian Rasche9Institute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyInstitute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyDepartment of Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Leipzig, Leipzig, GermanyInstitute of Immunology, University of Heidelberg, Heidelberg, GermanyCenter for Geriatric Medicine, Bezirksklinikum Regensburg, Regensburg, GermanyMedical Department I, Nephrology Division, University Hospital Ulm, Ulm, GermanyDepartment of Nephrology, KfH Renal Unit, Hospital St. Georg, Leipzig, GermanyInstitute for Organic Chemistry, University of Leipzig, Leipzig, GermanyInstitute of Clinical Immunology, Medical Faculty, University of Leipzig, Leipzig, GermanyDepartment of Internal Medicine, Neurology and Dermatology, Clinic for Endocrinology and Nephrology, Section of Nephrology, University Hospital Leipzig, Leipzig, GermanyBackground. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concentrations of TGFβ, sCD30 (ELISA), and interleukins (CBA) of fifteen patients eligible for renal transplantation were analyzed before, during, and after a regular HD. Results. Before HD, the median proportion of CD8+ effector cells, CD8+ CCR5+ effector cells, and HLA-DR+ regulatory T cells as well as the median concentration of soluble CD30 increased and naive CD8+ T cells decreased. During HD, there was a significant decrease in CD4- CD8- T cells (p<0.001) and an increase in CD25+ T cells (p=0.026), sCD30 (p<0.001), HLA-DR+ regulatory T cells (p=0.005), and regulatory T cells (p=0.003). TGFβ and sCD30 increased significantly over time. The activity of the classical complement pathway started to slightly increase after the first hour of HD and lasted until fifteen minutes after finishing dialysis. The decrease in the functional activity of the alternative pathway was only transient and was followed by a significant increase within 15 minutes after finishing the treatment. Conclusion. HD might interact with the allograft outcome by influencing T cell subsets and activation of the complement system in a biphasic course.http://dx.doi.org/10.1155/2019/8389765 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kristin Mai Andreas Boldt Hans-Michael Hau Michael Kirschfink Stephan Schiekofer Frieder Keller Joachim Beige Athanassios Giannis Ulrich Sack Franz Maximilian Rasche |
spellingShingle |
Kristin Mai Andreas Boldt Hans-Michael Hau Michael Kirschfink Stephan Schiekofer Frieder Keller Joachim Beige Athanassios Giannis Ulrich Sack Franz Maximilian Rasche Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation Analytical Cellular Pathology |
author_facet |
Kristin Mai Andreas Boldt Hans-Michael Hau Michael Kirschfink Stephan Schiekofer Frieder Keller Joachim Beige Athanassios Giannis Ulrich Sack Franz Maximilian Rasche |
author_sort |
Kristin Mai |
title |
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation |
title_short |
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation |
title_full |
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation |
title_fullStr |
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation |
title_full_unstemmed |
Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation |
title_sort |
immunological alterations due to hemodialysis might interfere with early complications in renal transplantation |
publisher |
Hindawi Limited |
series |
Analytical Cellular Pathology |
issn |
2210-7177 2210-7185 |
publishDate |
2019-01-01 |
description |
Background. Chronic or intercurrent alterations of the immune system in patients with end-stage renal disease (CKD) and intermittent hemodialysis (CKD5D, HD) have been attributed to an acute rejection of renal allograft. Methods. Leukocyte subsets in flow cytometry, complement activation, and concentrations of TGFβ, sCD30 (ELISA), and interleukins (CBA) of fifteen patients eligible for renal transplantation were analyzed before, during, and after a regular HD. Results. Before HD, the median proportion of CD8+ effector cells, CD8+ CCR5+ effector cells, and HLA-DR+ regulatory T cells as well as the median concentration of soluble CD30 increased and naive CD8+ T cells decreased. During HD, there was a significant decrease in CD4- CD8- T cells (p<0.001) and an increase in CD25+ T cells (p=0.026), sCD30 (p<0.001), HLA-DR+ regulatory T cells (p=0.005), and regulatory T cells (p=0.003). TGFβ and sCD30 increased significantly over time. The activity of the classical complement pathway started to slightly increase after the first hour of HD and lasted until fifteen minutes after finishing dialysis. The decrease in the functional activity of the alternative pathway was only transient and was followed by a significant increase within 15 minutes after finishing the treatment. Conclusion. HD might interact with the allograft outcome by influencing T cell subsets and activation of the complement system in a biphasic course. |
url |
http://dx.doi.org/10.1155/2019/8389765 |
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