Detection of pathogenic bacteria in diarrheal stool collected from children in North Lebanon by using conventional stool culture and microarray technique « CLART® Enterobac »

• Main Authors: Ranin Bechara, Michel Hosny, Imad AL-Kassaa, Fouad Dabboussi, Hassan Mallat, Monzer Hamze
• Format: Article
• Language: English
• Published: National Council for Scientific Research-Lebanon 2016-12-01
• Series: Lebanese Science Journal
• Subjects: Enteropathogens; CLART® EnteroBac; stool culture
• Online Access: http://lsj.cnrs.edu.lb/2016/12/30/r-bechara-m-hosny-i-al-kassaa-f-dabboussi-h-mallat-and-m-hamze/

Bechara, R., Hosny, M., AL-Kassaa, I., Dabboussi, F., Mallat, H. and Hamze, M. 2016. Detection of pathogenic bacteria in diarrheal stool collected from children in north lebanon by using conventional stool culture and microarray technique « clart® enterobac ». Lebanese Science Journal, 17(2): 233-239. Illness caused by enteropathogens represents an important economic and health burden worldwide. The majority of enteropathogens causes gastrointestinal infections which have a great impact on public health both in developing and developed countries. The aim of this study is to detect and identify the main enteropathogens in Lebanese diarrheal stool from children under 15 years old. The detection was performed by using both conventional method and microarray technique CLART® EnteroBac (Genomica-Spain). Five enteric pathogens, Salmonella spp, Shigella spp, Clostridium difficile B, Enteropathogenic E. coli, Campylobacter jejuni were detected in 80 diarrheal stools, from children under 15 years old. The results showed that CLART® EnteroBac technique have detected enteropathogens in 19% of samples, whereas 1% returned positive using stool culture methods.