Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

Abstract Background Application of the CRISPR/Cas9 system or its derived base editors enables targeted genome modification, thereby providing a programmable tool to exploit gene functions and to improve crop traits. Results We report that PmCDA1 is much more efficient than rAPOBEC1 when fused to CRI...

Full description

Bibliographic Details
Main Authors: Wen Xu, Wei Song, Yongxing Yang, Ying Wu, Xinxin Lv, Shuang Yuan, Ya Liu, Jinxiao Yang
Format: Article
Language:English
Published: BMC 2019-11-01
Series:BMC Plant Biology
Subjects:
CRISPR/Cas9
Base editing
High-fidelity Cas9 variants
tRNA-sgRNA
Off-target effect
Online Access:http://link.springer.com/article/10.1186/s12870-019-2131-1

Internet

http://link.springer.com/article/10.1186/s12870-019-2131-1

Similar Items