Protection Efficacy of Multivalent Egg Yolk Immunoglobulin against Eimeria tenella Infection in Chickens

• Main Authors: Jj Xu, Cz Ren, Ss Wang, Dd Liu, Lq Cao, Jp Tao
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2013-09-01
• Series: Iranian Journal of Parasitology
• Subjects: Coccidiosis; Eimeria tenella; IgY; Passive immunization
• Online Access: https://ijpa.tums.ac.ir/index.php/ijpa/article/view/470
• ISSN: 1735-7020; 2008-238X

  Background: To control avian coccidiosis with drug-independent strategy effec-tively and safely, multivalent hyperimmune egg yolk immunoglobulin (IgY) was prepared and its ability to protect against Eimeria tenella infection was evaluated. Methods: Hens were orally immunized with live oocysts of 5 species of Eimeria for six times, antibody titers in serum and yolk were monitored by indirect enzyme-linked immunosorbent assay. The specific IgY was isolated, purified and lyophi-lized. IgY powder was orally administrated as dietary supplement in newly hatched chicks at various dosages. Birds were orally challenged with 10000 sporulated oo-cysts of E. tenella at 10 days of age, weighed and killed at 8 days post challenge, and the protective effect was assessed. Results: The averge yeid of IgY was 9.2 mg/ml yolk, the antibody titer of IgY reached to 1:163840 per mg with the purity up to 98%. Chickens fed IgY resulted in reduced mortality, increased body weight gain (BWG), reduced oocyst shedding, reduced caecal lesion score and increased anti-coccidial index. In terms of BWG and caecal lesion, IgY significantly enhanced the resistance of bird at ≥ 0.05% of IgY in the diet when compared with the challenged control group (P<0.05). No significant difference was observed at dosage ≥ 0.5% and 1.0% when BWG and caecal lesion were compared with the sodium salinomycin control group, respec-tively (P>0.05). Conclusion: Supplementing newly hatched chicks with Eimeria-specific IgY repre-sents a promising strategy to prevent avian coccidiosis.