Generation and characterization of two iPSC lines from human epicardium-derived cells

Generation and characterization of two iPSC lines from human epicardium-derived cells

Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analys...

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Main Authors: Christina Paulitschek, Peggy Schulze-Matz, Julia Hesse, Timo Schmidt, Wasco Wruck, James Adjaye, Jürgen Schrader
Format: Article
Language:English
Published: Elsevier 2017-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117300211
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spelling doaj-ee1e7b05f8114275b281e35f023729932020-11-25T00:17:40ZengElsevierStem Cell Research1873-50611876-77532017-04-0120C505310.1016/j.scr.2017.02.007Generation and characterization of two iPSC lines from human epicardium-derived cellsChristina Paulitschek0Peggy Schulze-Matz1Julia Hesse2Timo Schmidt3Wasco Wruck4James Adjaye5Jürgen Schrader6Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyInstitute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyDepartment of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyDepartment of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyInstitute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyInstitute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyDepartment of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, GermanyHuman epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.http://www.sciencedirect.com/science/article/pii/S1873506117300211
collection DOAJ
language English
format Article
sources DOAJ
author Christina Paulitschek
Peggy Schulze-Matz
Julia Hesse
Timo Schmidt
Wasco Wruck
James Adjaye
Jürgen Schrader
spellingShingle Christina Paulitschek
Peggy Schulze-Matz
Julia Hesse
Timo Schmidt
Wasco Wruck
James Adjaye
Jürgen Schrader
Generation and characterization of two iPSC lines from human epicardium-derived cells
Stem Cell Research
author_facet Christina Paulitschek
Peggy Schulze-Matz
Julia Hesse
Timo Schmidt
Wasco Wruck
James Adjaye
Jürgen Schrader
author_sort Christina Paulitschek
title Generation and characterization of two iPSC lines from human epicardium-derived cells
title_short Generation and characterization of two iPSC lines from human epicardium-derived cells
title_full Generation and characterization of two iPSC lines from human epicardium-derived cells
title_fullStr Generation and characterization of two iPSC lines from human epicardium-derived cells
title_full_unstemmed Generation and characterization of two iPSC lines from human epicardium-derived cells
title_sort generation and characterization of two ipsc lines from human epicardium-derived cells
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2017-04-01
description Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.
url http://www.sciencedirect.com/science/article/pii/S1873506117300211
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