Generation and characterization of two iPSC lines from human epicardium-derived cells

Generation and characterization of two iPSC lines from human epicardium-derived cells

Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analys...

Full description

Bibliographic Details
Main Authors: Christina Paulitschek, Peggy Schulze-Matz, Julia Hesse, Timo Schmidt, Wasco Wruck, James Adjaye, Jürgen Schrader
Format: Article
Language:English
Published: Elsevier 2017-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117300211
Description
Summary:Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.
ISSN:1873-5061
1876-7753

Similar Items