Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
Liver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divid...
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2020-02-01
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doaj-ee0e68b5993540d1b982e23ac809e5ac2020-11-25T02:09:56ZengIslamic Azad UniversityJournal of Chemical Health Risks2251-67192251-67272020-02-01101455610.22034/jchr.2020.1881598.1058671481Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in RatsAhmed Talaat Abd Elaziz0Hanaa Mohamed Elzahed1Shar Hassan Hassan2Bahaa Eldin A. Khaled3Eman H. Nadwa4Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, EgyptForensic Medicine Department, Faculty of Medicine, Cairo University, Cairo, EgyptLab Technology Department, Faculty of Applied Medical Science, Misr University For Science &Technology, Cairo, Egypt.4Department of Anatomy, College of Medicine, Jouf University, Jouf, Arab SaudiDepartment of Anatomy, College of Medicine, Jouf University, Jouf, Arab Saudi Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Pharmcology and Therapeutics, College of Medicine, Jouf University, Jouf, Arab Saudi Department of Medical pharmacology, Faculty of Medicine, Cairo University, Cairo, EgyptLiver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divided into seven groups (n=10 rats/group) as follows, group1: control rats, group 2: CCL<sub>4</sub>, group 3: CCL<sub>4</sub> that received MSCs group 4: CCL<sub>4</sub> that received HO-1 inhibitor, group 5: CCL<sub>4</sub> that received HO-1 inducer, group 6: CCL<sub>4</sub> that received combined MSCs and HO-1 inhibitor , and group 7: CCL<sub>4</sub> that received combined MSCs and HO-1 inducer. All groups were evaluated histopathologically with assessment of liver functions. The combined MSCs and HO-1 inducer group showed the highest significant results in ALT (p-value ˂0.05), albumin (p-value ˂0.05), HO-1 activity (p-value ˂0.0001), and genes expression compared to other groups. This is due to the cumulative anti-inflammatory role of both MSCs and HO-1 together with the ability of MSCs to increase the HO-1 expression with further reduction in inflammation and fibrosis. MSCs and HO-1 inducer provide promising tool in treatment of liver disease.http://www.jchr.org/article_671481_7bca0f9f8fc34a5e4ff15257e0507c62.pdfmscsliver fibrosisho-1 inducerho-1 inhibitor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Talaat Abd Elaziz Hanaa Mohamed Elzahed Shar Hassan Hassan Bahaa Eldin A. Khaled Eman H. Nadwa |
spellingShingle |
Ahmed Talaat Abd Elaziz Hanaa Mohamed Elzahed Shar Hassan Hassan Bahaa Eldin A. Khaled Eman H. Nadwa Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats Journal of Chemical Health Risks mscs liver fibrosis ho-1 inducer ho-1 inhibitor |
author_facet |
Ahmed Talaat Abd Elaziz Hanaa Mohamed Elzahed Shar Hassan Hassan Bahaa Eldin A. Khaled Eman H. Nadwa |
author_sort |
Ahmed Talaat Abd Elaziz |
title |
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats |
title_short |
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats |
title_full |
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats |
title_fullStr |
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats |
title_full_unstemmed |
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats |
title_sort |
cumulative effect of mesenchymal stem cells and heme oxygenase-1 inducer in ameliorating induced liver toxicity in rats |
publisher |
Islamic Azad University |
series |
Journal of Chemical Health Risks |
issn |
2251-6719 2251-6727 |
publishDate |
2020-02-01 |
description |
Liver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divided into seven groups (n=10 rats/group) as follows, group1: control rats, group 2: CCL<sub>4</sub>, group 3: CCL<sub>4</sub> that received MSCs group 4: CCL<sub>4</sub> that received HO-1 inhibitor, group 5: CCL<sub>4</sub> that received HO-1 inducer, group 6: CCL<sub>4</sub> that received combined MSCs and HO-1 inhibitor , and group 7: CCL<sub>4</sub> that received combined MSCs and HO-1 inducer. All groups were evaluated histopathologically with assessment of liver functions. The combined MSCs and HO-1 inducer group showed the highest significant results in ALT (p-value ˂0.05), albumin (p-value ˂0.05), HO-1 activity (p-value ˂0.0001), and genes expression compared to other groups. This is due to the cumulative anti-inflammatory role of both MSCs and HO-1 together with the ability of MSCs to increase the HO-1 expression with further reduction in inflammation and fibrosis. MSCs and HO-1 inducer provide promising tool in treatment of liver disease. |
topic |
mscs liver fibrosis ho-1 inducer ho-1 inhibitor |
url |
http://www.jchr.org/article_671481_7bca0f9f8fc34a5e4ff15257e0507c62.pdf |
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