Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats

Liver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divid...

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Main Authors: Ahmed Talaat Abd Elaziz, Hanaa Mohamed Elzahed, Shar Hassan Hassan, Bahaa Eldin A. Khaled, Eman H. Nadwa
Format: Article
Language:English
Published: Islamic Azad University 2020-02-01
Series:Journal of Chemical Health Risks
Subjects:
Online Access:http://www.jchr.org/article_671481_7bca0f9f8fc34a5e4ff15257e0507c62.pdf
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spelling doaj-ee0e68b5993540d1b982e23ac809e5ac2020-11-25T02:09:56ZengIslamic Azad UniversityJournal of Chemical Health Risks2251-67192251-67272020-02-01101455610.22034/jchr.2020.1881598.1058671481Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in RatsAhmed Talaat Abd Elaziz0Hanaa Mohamed Elzahed1Shar Hassan Hassan2Bahaa Eldin A. Khaled3Eman H. Nadwa4Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, EgyptForensic Medicine Department, Faculty of Medicine, Cairo University, Cairo, EgyptLab Technology Department, Faculty of Applied Medical Science, Misr University For Science &Technology, Cairo, Egypt.4Department of Anatomy, College of Medicine, Jouf University, Jouf, Arab SaudiDepartment of Anatomy, College of Medicine, Jouf University, Jouf, Arab Saudi Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Pharmcology and Therapeutics, College of Medicine, Jouf University, Jouf, Arab Saudi Department of Medical pharmacology, Faculty of Medicine, Cairo University, Cairo, EgyptLiver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divided into seven groups (n=10 rats/group) as follows, group1: control rats, group 2: CCL<sub>4</sub>, group 3: CCL<sub>4</sub> that received MSCs group 4: CCL<sub>4</sub> that received HO-1 inhibitor, group 5: CCL<sub>4</sub> that received HO-1 inducer, group 6: CCL<sub>4</sub> that received combined MSCs and HO-1 inhibitor , and group 7: CCL<sub>4</sub> that received combined MSCs and HO-1 inducer. All groups were evaluated histopathologically with assessment of liver functions. The combined MSCs and HO-1 inducer group showed the highest significant results in ALT (p-value ˂0.05), albumin (p-value ˂0.05), HO-1 activity (p-value ˂0.0001), and genes expression compared to other groups.  This is due to the cumulative anti-inflammatory role of both MSCs and HO-1 together with the ability of MSCs to increase the HO-1 expression with further reduction in inflammation and fibrosis. MSCs and HO-1 inducer provide promising tool in treatment of liver disease.http://www.jchr.org/article_671481_7bca0f9f8fc34a5e4ff15257e0507c62.pdfmscsliver fibrosisho-1 inducerho-1 inhibitor
collection DOAJ
language English
format Article
sources DOAJ
author Ahmed Talaat Abd Elaziz
Hanaa Mohamed Elzahed
Shar Hassan Hassan
Bahaa Eldin A. Khaled
Eman H. Nadwa
spellingShingle Ahmed Talaat Abd Elaziz
Hanaa Mohamed Elzahed
Shar Hassan Hassan
Bahaa Eldin A. Khaled
Eman H. Nadwa
Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
Journal of Chemical Health Risks
mscs
liver fibrosis
ho-1 inducer
ho-1 inhibitor
author_facet Ahmed Talaat Abd Elaziz
Hanaa Mohamed Elzahed
Shar Hassan Hassan
Bahaa Eldin A. Khaled
Eman H. Nadwa
author_sort Ahmed Talaat Abd Elaziz
title Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
title_short Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
title_full Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
title_fullStr Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
title_full_unstemmed Cumulative Effect of Mesenchymal Stem Cells and Heme Oxygenase-1 Inducer in Ameliorating Induced Liver Toxicity in Rats
title_sort cumulative effect of mesenchymal stem cells and heme oxygenase-1 inducer in ameliorating induced liver toxicity in rats
publisher Islamic Azad University
series Journal of Chemical Health Risks
issn 2251-6719
2251-6727
publishDate 2020-02-01
description Liver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divided into seven groups (n=10 rats/group) as follows, group1: control rats, group 2: CCL<sub>4</sub>, group 3: CCL<sub>4</sub> that received MSCs group 4: CCL<sub>4</sub> that received HO-1 inhibitor, group 5: CCL<sub>4</sub> that received HO-1 inducer, group 6: CCL<sub>4</sub> that received combined MSCs and HO-1 inhibitor , and group 7: CCL<sub>4</sub> that received combined MSCs and HO-1 inducer. All groups were evaluated histopathologically with assessment of liver functions. The combined MSCs and HO-1 inducer group showed the highest significant results in ALT (p-value ˂0.05), albumin (p-value ˂0.05), HO-1 activity (p-value ˂0.0001), and genes expression compared to other groups.  This is due to the cumulative anti-inflammatory role of both MSCs and HO-1 together with the ability of MSCs to increase the HO-1 expression with further reduction in inflammation and fibrosis. MSCs and HO-1 inducer provide promising tool in treatment of liver disease.
topic mscs
liver fibrosis
ho-1 inducer
ho-1 inhibitor
url http://www.jchr.org/article_671481_7bca0f9f8fc34a5e4ff15257e0507c62.pdf
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