A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.

A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.

An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure e...

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Bibliographic Details
Main Authors: J C Khoo, E Miller, D I Goldberg
Format: Article
Language:English
Published: Elsevier 1987-07-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520386545
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spelling doaj-edffbec23a754e428c00e1386b601ab22021-04-25T04:20:22ZengElsevierJournal of Lipid Research0022-22751987-07-01287873877A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.J C KhooE MillerD I GoldbergAn enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.http://www.sciencedirect.com/science/article/pii/S0022227520386545
collection DOAJ
language English
format Article
sources DOAJ
author J C Khoo
E Miller
D I Goldberg
spellingShingle J C Khoo
E Miller
D I Goldberg
A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
Journal of Lipid Research
author_facet J C Khoo
E Miller
D I Goldberg
author_sort J C Khoo
title A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
title_short A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
title_full A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
title_fullStr A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
title_full_unstemmed A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
title_sort rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1987-07-01
description An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.
url http://www.sciencedirect.com/science/article/pii/S0022227520386545
work_keys_str_mv AT jckhoo arapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
AT emiller arapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
AT digoldberg arapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
AT jckhoo rapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
AT emiller rapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
AT digoldberg rapidandsensitiveenzymaticradiochemicalassayforthedeterminationoftriglycerides
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