[PROVISIONAL] Genome-wide identification and characterization of R2R3 MYB genes in Medicago truncatula

• Main Authors: Wei Li, Ying Liu, Jinyue Zhao, Xin Zhen, Changhong Guo, Yongjun Shu
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Genética
• Series: Genetics and Molecular Biology
• Subjects: Medicago truncatula; MYB; abiotic stress; qRT-PCR
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005022103&lng=en&tlng=en

Abstract MYB is a large family of plant transcription factors , its function has been identified in several plants, while few reports in Medicago truncatula. In this study, we used RNA seq data to analyze and identif y R2R3 MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. O ut of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data was used for functional analysis of the MtMYB genes during growth and development al processes providing e vidence for a role in tissue s differentiation, seed development processes and especially the nodulation process. Furthermore, we have investigated the expression of MtMYB genes in response to abiotic stresses using RNA-seq data, which confirmed the critical roles in signal transduction and regulation processes under abiotic stress. We used quantitative real time PCR (qRT PCR) to validate expression profiles. The expression pattern of M. truncatula MYB genes under different abiotic stress conditions suggest that some may play a major r ole in cross talk among different signal transduction pathways in response to abiotic stresses. Our study will serve as a foundation for future research into the molecular function of M. truncatula R2R3 MYB genes.