Protective Effects of Manganese on the Testis Structure and Sperm Parameters of Formalin-Treated Mice

• Main Authors: Shiva Tajaddini, Soheila Ebrahimi, Peymaneh Shirinbayan, Mehrdad Bakhtiyari, Babak Behnam, Mohamad Taghi Joghataei, Morteza Koruji
• Format: Article
• Language: fas
• Published: Vesnu Publications 2013-08-01
• Series: مجله دانشکده پزشکی اصفهان
• Subjects: Formaldehyde; Manganese; Sperm parameters; Testicular structure
• Online Access: http://jims.mui.ac.ir/index.php/jims/article/view/2640
• ISSN: 1027-7595; 1735-854X

Background: Formaldehyde, as a ubiquitous environmental pollutant, is extensively used in industrial and hospitals settings. The aim of this study was to investigate the protective role of manganese on testis structure and sperm parameters in adult mice exposed to formaldehyde (FA). Methods: After dose optimizing for FA and manganese chloride, twenty adult male NMRI mice were selected and randomly divided into four groups: (i) control, (ii) sham, (iii) FA-exposed, and (iv) FA and manganese chloride exposed. The FA-exposed groups received 10 mg/ kg FA daily for 14 days, and 5 mg/kg manganese chloride was just injected intraperitoneally on 2nd week. Mice were sacrificed, and spermatozoa were collected from the cauda of the right epididymis and analyzed for count, motility, morphology and viability. The other testicular tissues were weighed and prepared for histological examination upon removal. Seminiferous tubules, lumen diameters and epithelium thickness were also measured. Findings: The findings revealed that FA significantly reduced the testicular weight, sperm count, motility, viability and normal morphology compared with control group (P ≤ 0.05). In addition, seminiferous tubules atrophied and seminiferous epithelial cells disintegrated in the FA group in comparison with the control group (P ≤ 0.05). However, manganese improved the testicular structure and sperm parameters in FA-treated mice testes (P ≤ 0.05). Conclusion: According to the results, manganese has protective effects on mice epididymal sperm parameters and testis structure treated with FA.