METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN

Aim. To study the spectrum and level of short-chained fatty acids (SCFA) in supernatant of bifidobacteria under different microecological conditions of the colon biotope in human. Materials and methods. Metabolites of 88 bifidobacteria strains isolated from patients when examined for dysbiosis of th...

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Main Authors: O. V. Bukharin, E. V. Ivanova, N. B. Perunova, I. N. Chainikova, S. V. Andryuschenko
Format: Article
Language:Russian
Published: Central Research Institute for Epidemiology 2017-02-01
Series: Журнал микробиологии, эпидемиологии и иммунобиологии
Subjects:
Online Access:https://microbiol.elpub.ru/jour/article/view/116
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spelling doaj-edb254262d834ff395a34ac22df49c532021-07-28T13:07:49ZrusCentral Research Institute for Epidemiology Журнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132017-02-010131110.36233/0372-9311-2017-1-3-11116METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMANO. V. Bukharin0E. V. Ivanova1N. B. Perunova2I. N. Chainikova3S. V. Andryuschenko4Institute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisAim. To study the spectrum and level of short-chained fatty acids (SCFA) in supernatant of bifidobacteria under different microecological conditions of the colon biotope in human. Materials and methods. Metabolites of 88 bifidobacteria strains isolated from patients when examined for dysbiosis of the colon were investigated. Definition of concentration of SCFA was performed on acidified supernatant samples by a separation method on chromatograph GC-2010 Plus, Shimadzu (Japan). Results. Monobasic acids were found in metabolites of 50 -100% study cultures of bifidobacteria where the spectrum and level of carboxylic acids in supernatants varied depending on microecological condition of the origin of the discharge. In severe damages of microsym-biocenosis in metabolites of Bifidobacterium spp., summarized concentrations of SCFA, structural index, levels of aceitic and propionic acids were decreased. Strain-specific differences in a metabolic profile of bifidoflora in a composition of individual consortiums were determined. Data obtained indicate the variation of functional (metabolic) activity of dominant strains in different microecological conditions of the human colon. Conclusion. Uniquieness of metabolome of every other strain due to their strain specifity determines their functional activity, but a metabolic profile of bifidoflora can serve as the most important criterion for the selection of effective probiotic drugs for treatment and prevention of dysbioisis in the colon.https://microbiol.elpub.ru/jour/article/view/116bifidobacteriashort-chained fatty acidsmetabolic profilestrain specifitydysbiosis
collection DOAJ
language Russian
format Article
sources DOAJ
author O. V. Bukharin
E. V. Ivanova
N. B. Perunova
I. N. Chainikova
S. V. Andryuschenko
spellingShingle O. V. Bukharin
E. V. Ivanova
N. B. Perunova
I. N. Chainikova
S. V. Andryuschenko
METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
Журнал микробиологии, эпидемиологии и иммунобиологии
bifidobacteria
short-chained fatty acids
metabolic profile
strain specifity
dysbiosis
author_facet O. V. Bukharin
E. V. Ivanova
N. B. Perunova
I. N. Chainikova
S. V. Andryuschenko
author_sort O. V. Bukharin
title METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
title_short METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
title_full METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
title_fullStr METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
title_full_unstemmed METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN
title_sort metabolic profile of bifidoflora under different microeco-logical conditions of the colon biotope in human
publisher Central Research Institute for Epidemiology
series Журнал микробиологии, эпидемиологии и иммунобиологии
issn 0372-9311
2686-7613
publishDate 2017-02-01
description Aim. To study the spectrum and level of short-chained fatty acids (SCFA) in supernatant of bifidobacteria under different microecological conditions of the colon biotope in human. Materials and methods. Metabolites of 88 bifidobacteria strains isolated from patients when examined for dysbiosis of the colon were investigated. Definition of concentration of SCFA was performed on acidified supernatant samples by a separation method on chromatograph GC-2010 Plus, Shimadzu (Japan). Results. Monobasic acids were found in metabolites of 50 -100% study cultures of bifidobacteria where the spectrum and level of carboxylic acids in supernatants varied depending on microecological condition of the origin of the discharge. In severe damages of microsym-biocenosis in metabolites of Bifidobacterium spp., summarized concentrations of SCFA, structural index, levels of aceitic and propionic acids were decreased. Strain-specific differences in a metabolic profile of bifidoflora in a composition of individual consortiums were determined. Data obtained indicate the variation of functional (metabolic) activity of dominant strains in different microecological conditions of the human colon. Conclusion. Uniquieness of metabolome of every other strain due to their strain specifity determines their functional activity, but a metabolic profile of bifidoflora can serve as the most important criterion for the selection of effective probiotic drugs for treatment and prevention of dysbioisis in the colon.
topic bifidobacteria
short-chained fatty acids
metabolic profile
strain specifity
dysbiosis
url https://microbiol.elpub.ru/jour/article/view/116
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