Rapid detection of Yersinia enterocolitica by using Real-Time PCR technique in some types of foods in Al-Qadisiyah province

• Main Author: Kadhim H. Abbas
• Format: Article
• Language: English
• Published: College of Veterinary Medicine 2015-06-01
• Series: Al-Qadisiyah Journal of Veterinary Medicine Sciences
• Online Access: http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/323
• ISSN: 1818-5746; 2313-4429

The present study was carried out for direct and rapid detection of pathogenic Y. enterocolitica in some types of foods such as (raw milk, local sweet cheese, and minced beef meat) exist in local different markets of Al-Qadisiya province. A total of 192 samples were collected randomly from markets of Al-Qadisiya province involved 79 raw milk samples, 58 local sweet cheese samples, and 55 minced beef meat samples. Real-Time PCR technique based SYBER Green dye were used for detection of this bacteria by amplifying invasion locus protein (ail) gene which is found especially in Y. enterocolitica, The results revealed that positive samples for this (ail) gene were 49 samples out of 79 examined raw milk (62%), also 6 samples out of 58 samples observed in local sweet cheese (10.3%) and 49 samples out of 55 samples of minced beef meat (89%). A total of positive samples were 104 out of 192 examined samples, samples of minced beef meat recorded highly significant contamination (p<0.05) as compared samples of raw milk which occupied the second rank in contamination with a pathogen and then samples of local sweet cheese which recorded less degree of contamination. The Real-time PCR technique was very specific and efficient for detection Y. enterocolitica in a variety of foods, when compared with culture method which has many problems.