Response surface methodology of nitrilase production by recombinant Escherichia coli

Response surface methodology of nitrilase production by recombinant Escherichia coli

Growth and nitrilase production by recombinant Escherichia coli cells harbouring pET 21 (b) plasmid, for the expression of Pseudomonas putida nitrilase were improved using response surface methodology. Central composite design was used for obtaining ideal concentration of critical medium components...

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Bibliographic Details
Main Authors:	Sachin Dubey, Amit Singh, Uttam C Banerjee
Format:	Article
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2011-09-01
Series:	Brazilian Journal of Microbiology
Subjects:	Biocatalysis Recombinant E. coli Nitrilase Response surface methodology Central composite design
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300029

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