Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation

The lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin−a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indol...

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Main Authors:	Vladislav V. Yemelyanov, Victor V. Lastochkin, Tamara V. Chirkova, Sylvia M. Lindberg, Maria F. Shishova
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Biomolecules
Subjects:	auxin oxygen deficiency post-anoxia rice tobacco wheat
Online Access:	https://www.mdpi.com/2218-273X/10/2/276

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spelling	doaj-ed8ccf5bf2c04bb1aaa442a1cbbef9ed2020-11-25T02:03:34ZengMDPI AGBiomolecules2218-273X2020-02-0110227610.3390/biom10020276biom10020276Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent ReoxygenationVladislav V. Yemelyanov0Victor V. Lastochkin1Tamara V. Chirkova2Sylvia M. Lindberg3Maria F. Shishova4Department of Genetics and Biotechnology, Saint-Petersburg State University, Universitetskaya em., 7/9, 199034 Saint-Petersburg, RussiaDepartment of Plant Physiology and Biochemistry, Saint-Petersburg State University, Universitetskaya em., 7/9, 199034 Saint-Petersburg, RussiaDepartment of Plant Physiology and Biochemistry, Saint-Petersburg State University, Universitetskaya em., 7/9, 199034 Saint-Petersburg, RussiaDepartment of Ecology, Environment and Plant Sciences, Stockholm University, SE-106 91, Stockholm, SwedenDepartment of Plant Physiology and Biochemistry, Saint-Petersburg State University, Universitetskaya em., 7/9, 199034 Saint-Petersburg, RussiaThe lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin−a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indoleacetic acid (IAA) content in plants was measured by ELISA. The obtained data revealed anoxia-induced accumulation of IAA in wheat and rice seedlings related to their tolerance of oxygen deprivation. The highest IAA accumulation was detected in rice roots. Subsequent reoxygenation was accompanied with a fast auxin reduction to the control level. A major difference was reported for shoots: wheat seedlings contained less than one-third of normoxic level of auxin during post-anoxia, while IAA level in rice seedlings rapidly recovered to normoxic level. It is likely that the mechanisms of auxin dynamics resulted from oxygen-induced shift in auxin degradation and transport. Exogenous IAA treatment enhanced plant survival under anoxia by decreased electrolyte leakage, production of hydrogen peroxide and lipid peroxidation. The positive effect of external IAA application coincided with improvement of tolerance to oxygen deprivation in the 35S:<i>iaaM</i> × 35S:<i>iaaH</i> lines of transgene tobacco due to its IAA overproduction.https://www.mdpi.com/2218-273X/10/2/276auxinoxygen deficiencypost-anoxiaricetobaccowheat
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Vladislav V. Yemelyanov Victor V. Lastochkin Tamara V. Chirkova Sylvia M. Lindberg Maria F. Shishova
spellingShingle	Vladislav V. Yemelyanov Victor V. Lastochkin Tamara V. Chirkova Sylvia M. Lindberg Maria F. Shishova Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation Biomolecules auxin oxygen deficiency post-anoxia rice tobacco wheat
author_facet	Vladislav V. Yemelyanov Victor V. Lastochkin Tamara V. Chirkova Sylvia M. Lindberg Maria F. Shishova
author_sort	Vladislav V. Yemelyanov
title	Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation
title_short	Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation
title_full	Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation
title_fullStr	Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation
title_full_unstemmed	Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation
title_sort	indoleacetic acid levels in wheat and rice seedlings under oxygen deficiency and subsequent reoxygenation
publisher	MDPI AG
series	Biomolecules
issn	2218-273X
publishDate	2020-02-01
description	The lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin−a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indoleacetic acid (IAA) content in plants was measured by ELISA. The obtained data revealed anoxia-induced accumulation of IAA in wheat and rice seedlings related to their tolerance of oxygen deprivation. The highest IAA accumulation was detected in rice roots. Subsequent reoxygenation was accompanied with a fast auxin reduction to the control level. A major difference was reported for shoots: wheat seedlings contained less than one-third of normoxic level of auxin during post-anoxia, while IAA level in rice seedlings rapidly recovered to normoxic level. It is likely that the mechanisms of auxin dynamics resulted from oxygen-induced shift in auxin degradation and transport. Exogenous IAA treatment enhanced plant survival under anoxia by decreased electrolyte leakage, production of hydrogen peroxide and lipid peroxidation. The positive effect of external IAA application coincided with improvement of tolerance to oxygen deprivation in the 35S:<i>iaaM</i> × 35S:<i>iaaH</i> lines of transgene tobacco due to its IAA overproduction.
topic	auxin oxygen deficiency post-anoxia rice tobacco wheat
url	https://www.mdpi.com/2218-273X/10/2/276
work_keys_str_mv	AT vladislavvyemelyanov indoleaceticacidlevelsinwheatandriceseedlingsunderoxygendeficiencyandsubsequentreoxygenation AT victorvlastochkin indoleaceticacidlevelsinwheatandriceseedlingsunderoxygendeficiencyandsubsequentreoxygenation AT tamaravchirkova indoleaceticacidlevelsinwheatandriceseedlingsunderoxygendeficiencyandsubsequentreoxygenation AT sylviamlindberg indoleaceticacidlevelsinwheatandriceseedlingsunderoxygendeficiencyandsubsequentreoxygenation AT mariafshishova indoleaceticacidlevelsinwheatandriceseedlingsunderoxygendeficiencyandsubsequentreoxygenation
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Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation

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