Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome
Abstract Nucleo-cytoplasmic large DNA viruses are doubled stranded DNA viruses capable of infecting eukaryotic cells. Since the discovery of Mimivirus and Pandoravirus, there has been no doubt about their extraordinary features compared to “classic” viruses. Recently, we reported the expansion of th...
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-018-0976-9 |
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doaj-ed845e7522e64529b9e679aca1355a162020-11-25T00:14:20ZengBMCVirology Journal1743-422X2018-04-011511510.1186/s12985-018-0976-9Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenomeJulien Andreani0Jonathan Verneau1Didier Raoult2Anthony Levasseur3Bernard La Scola4Aix-Marseille Université, IRD, APHM, MEPHI, IHU Méditerranée InfectionAix-Marseille Université, IRD, APHM, MEPHI, IHU Méditerranée InfectionAix-Marseille Université, IRD, APHM, MEPHI, IHU Méditerranée InfectionAix-Marseille Université, IRD, APHM, MEPHI, IHU Méditerranée InfectionAix-Marseille Université, IRD, APHM, MEPHI, IHU Méditerranée InfectionAbstract Nucleo-cytoplasmic large DNA viruses are doubled stranded DNA viruses capable of infecting eukaryotic cells. Since the discovery of Mimivirus and Pandoravirus, there has been no doubt about their extraordinary features compared to “classic” viruses. Recently, we reported the expansion of the proposed family Pithoviridae, with the description of Cedratvirus and Orpheovirus, two new viruses related to Pithoviruses. Studying the major capsid protein of Orpheovirus, we detected a homologous sequence in a mine drainage metagenome. The in-depth exploration of this metagenome, using the MG-Digger program, enabled us to retrieve up to 10 contigs with clear evidence of viral sequences. Moreover, phylogenetic analyses further extended our screening with the discovery in another marine metagenome of a second virus closely related to Orpheovirus IHUMI-LCC2. This virus is a misidentified virus confused with and annotated as a Rickettsiales bacterium. It presents a partial genome size of about 170 kbp.http://link.springer.com/article/10.1186/s12985-018-0976-9PithovirusCedratvirusOrpheovirusMine drainage metagenomeMarine metagenomeMG-digger |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Julien Andreani Jonathan Verneau Didier Raoult Anthony Levasseur Bernard La Scola |
| spellingShingle |
Julien Andreani Jonathan Verneau Didier Raoult Anthony Levasseur Bernard La Scola Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome Virology Journal Pithovirus Cedratvirus Orpheovirus Mine drainage metagenome Marine metagenome MG-digger |
| author_facet |
Julien Andreani Jonathan Verneau Didier Raoult Anthony Levasseur Bernard La Scola |
| author_sort |
Julien Andreani |
| title |
Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| title_short |
Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| title_full |
Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| title_fullStr |
Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| title_full_unstemmed |
Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| title_sort |
deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome |
| publisher |
BMC |
| series |
Virology Journal |
| issn |
1743-422X |
| publishDate |
2018-04-01 |
| description |
Abstract Nucleo-cytoplasmic large DNA viruses are doubled stranded DNA viruses capable of infecting eukaryotic cells. Since the discovery of Mimivirus and Pandoravirus, there has been no doubt about their extraordinary features compared to “classic” viruses. Recently, we reported the expansion of the proposed family Pithoviridae, with the description of Cedratvirus and Orpheovirus, two new viruses related to Pithoviruses. Studying the major capsid protein of Orpheovirus, we detected a homologous sequence in a mine drainage metagenome. The in-depth exploration of this metagenome, using the MG-Digger program, enabled us to retrieve up to 10 contigs with clear evidence of viral sequences. Moreover, phylogenetic analyses further extended our screening with the discovery in another marine metagenome of a second virus closely related to Orpheovirus IHUMI-LCC2. This virus is a misidentified virus confused with and annotated as a Rickettsiales bacterium. It presents a partial genome size of about 170 kbp. |
| topic |
Pithovirus Cedratvirus Orpheovirus Mine drainage metagenome Marine metagenome MG-digger |
| url |
http://link.springer.com/article/10.1186/s12985-018-0976-9 |
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